Research output: Contribution to journal › Article › peer-review
Random Copolymers of Lysine and Isoleucine for Efficient mRNA Delivery. / Pilipenko, Iuliia; Korovkina, Olga; Gubina, Nina; Ekimova, Viktoria; Ishutinova, Anastasia; Korzhikova-Vlakh, Evgenia; Tennikova, Tatiana; Korzhikov-Vlakh, Viktor.
In: International Journal of Molecular Sciences, Vol. 23, No. 10, 5363, 11.05.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Random Copolymers of Lysine and Isoleucine for Efficient mRNA Delivery
AU - Pilipenko, Iuliia
AU - Korovkina, Olga
AU - Gubina, Nina
AU - Ekimova, Viktoria
AU - Ishutinova, Anastasia
AU - Korzhikova-Vlakh, Evgenia
AU - Tennikova, Tatiana
AU - Korzhikov-Vlakh, Viktor
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/5/11
Y1 - 2022/5/11
N2 - Messenger RNA (mRNA) is currently of great interest as a new category of therapeutic agent, which could be used for prevention or treatment of various diseases. For this mRNA requires effective delivery systems that will protect it from degradation, as well as allow cellular uptake and mRNA release. Random poly(lysine-co-isoleucine) polypeptides were synthesized and investigated as possible carriers for mRNA delivery. The polypeptides obtained under lysine:isoleucine monomer ratio equal to 80/20 were shown to give polyplexes with smaller size, positive ζ-potential and more than 90% encapsulation efficacy. The phase inversion method was proposed as best way for encapsulation of mRNA into polyplexes, which are based on obtained amphiphilic copolymers. These copolymers showed efficacy in protection of bound mRNA towards ribonuclease and lower toxicity as compared to lysine homopolymer. The poly(lysine-co-isoleucine) polypeptides showed greater than poly(ethyleneimine) efficacy as vectors for transfection of cells with green fluorescent protein and firefly luciferase encoding mRNAs. This allows us to consider obtained copolymers as promising candidates for mRNA delivery applications.
AB - Messenger RNA (mRNA) is currently of great interest as a new category of therapeutic agent, which could be used for prevention or treatment of various diseases. For this mRNA requires effective delivery systems that will protect it from degradation, as well as allow cellular uptake and mRNA release. Random poly(lysine-co-isoleucine) polypeptides were synthesized and investigated as possible carriers for mRNA delivery. The polypeptides obtained under lysine:isoleucine monomer ratio equal to 80/20 were shown to give polyplexes with smaller size, positive ζ-potential and more than 90% encapsulation efficacy. The phase inversion method was proposed as best way for encapsulation of mRNA into polyplexes, which are based on obtained amphiphilic copolymers. These copolymers showed efficacy in protection of bound mRNA towards ribonuclease and lower toxicity as compared to lysine homopolymer. The poly(lysine-co-isoleucine) polypeptides showed greater than poly(ethyleneimine) efficacy as vectors for transfection of cells with green fluorescent protein and firefly luciferase encoding mRNAs. This allows us to consider obtained copolymers as promising candidates for mRNA delivery applications.
KW - cationic polypeptides
KW - mRNA delivery
KW - polyplexes
KW - random amphiphilic copolymers
KW - transfection
UR - http://www.scopus.com/inward/record.url?scp=85129718788&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/b07dd551-1e26-37d6-8160-a4b5c140fc0c/
U2 - 10.3390/ijms23105363
DO - 10.3390/ijms23105363
M3 - Article
AN - SCOPUS:85129718788
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 10
M1 - 5363
ER -
ID: 95378365