Quantification of changes in cellular morphology during cell necrosis obtained from 3D refractive index distributions. / Gorbenko, D. A.; Belashov, A. V.; Belyaeva, T. N.; Kornilova, E. S.; Semenova, I. V.; Vasyutinskii, O. S.
In: Journal of Physics: Conference Series, Vol. 1236, No. 1, 012015, 16.07.2019.Research output: Contribution to journal › Conference article › peer-review
}
TY - JOUR
T1 - Quantification of changes in cellular morphology during cell necrosis obtained from 3D refractive index distributions
AU - Gorbenko, D. A.
AU - Belashov, A. V.
AU - Belyaeva, T. N.
AU - Kornilova, E. S.
AU - Semenova, I. V.
AU - Vasyutinskii, O. S.
N1 - Publisher Copyright: © Published under licence by IOP Publishing Ltd. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/7/16
Y1 - 2019/7/16
N2 - In this paper we perform an analysis of changes in cellular morphology in response to photodynamic treatment in vitro. Experiments were performed by means of digital holographic tomography. 3D spatial distributions of refractive index in cells were obtained and main morphological parameters of cells were determined: volume, average height, membrane area. The cellular response to treatment at different doses was studied and changes in morphology were correlated with major cell death pathways, apoptosis and necrosis. The necrosis pathway was studied in dynamics. The data obtained by holographic tomography were validated using a standard test for the integrity of cell membranes, conducted using a confocal fluorescent microscope.
AB - In this paper we perform an analysis of changes in cellular morphology in response to photodynamic treatment in vitro. Experiments were performed by means of digital holographic tomography. 3D spatial distributions of refractive index in cells were obtained and main morphological parameters of cells were determined: volume, average height, membrane area. The cellular response to treatment at different doses was studied and changes in morphology were correlated with major cell death pathways, apoptosis and necrosis. The necrosis pathway was studied in dynamics. The data obtained by holographic tomography were validated using a standard test for the integrity of cell membranes, conducted using a confocal fluorescent microscope.
UR - http://www.scopus.com/inward/record.url?scp=85069471942&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1236/1/012015
DO - 10.1088/1742-6596/1236/1/012015
M3 - Conference article
AN - SCOPUS:85069471942
VL - 1236
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
SN - 1742-6588
IS - 1
M1 - 012015
T2 - International Conference on Emerging Trends in Applied and Computational Physics 2019, ETACP 2019
Y2 - 21 March 2019 through 22 March 2019
ER -
ID: 76657655