Proteomic Profiling of the Human Fetal Multipotent Mesenchymal Stromal Cells Secretome. / Lobov, Arseniy A.; Yudintceva, Natalia M.; Mittenberg, Alexey G.; Shabelnikov, Sergey V.; Mikhailova, Natalia A.; Malashicheva, Anna B.; Khotin, Mikhail G.
In: Molecules (Basel, Switzerland), Vol. 25, No. 22, 5283, 12.11.2020.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Proteomic Profiling of the Human Fetal Multipotent Mesenchymal Stromal Cells Secretome
AU - Lobov, Arseniy A.
AU - Yudintceva, Natalia M.
AU - Mittenberg, Alexey G.
AU - Shabelnikov, Sergey V.
AU - Mikhailova, Natalia A.
AU - Malashicheva, Anna B.
AU - Khotin, Mikhail G.
N1 - Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2020/11/12
Y1 - 2020/11/12
N2 - Secretome of multipotent mesenchymal stromal cells (MSCs) is actively used in biomedical applications such as alveolar bone regeneration, treatment of cardiovascular disease, and neurodegenerative disorders. Nevertheless, hMSCs have low proliferative potential and production of the industrial quantity of their secretome might be challenging. Human fetal multipotent mesenchymal stromal cells (FetMSCs) isolated from early human embryo bone marrow are easy to expand and might be a potential source for pharmaceutical substances production based on their secretome. However, the secretome of FetMSCs was not previously analyzed. Here, we describe the secretome of FetMSCs using LC-MALDI shotgun proteomics. We identified 236 proteins. Functional annotation of the identified proteins revealed their involvement in angiogenesis, ossification, regulation of apoptosis, and immune response processes, which made it promising for biomedical applications. The proteins identified in the FetMSCs secretome are involved in the same biological processes as proteins from previously described adult hMSCs secretomes. Nevertheless, many of the common hMSCs secretome components (such as VEGF, FGF, Wnt and TGF-β) have not been identified in the FetMSCs secretome.
AB - Secretome of multipotent mesenchymal stromal cells (MSCs) is actively used in biomedical applications such as alveolar bone regeneration, treatment of cardiovascular disease, and neurodegenerative disorders. Nevertheless, hMSCs have low proliferative potential and production of the industrial quantity of their secretome might be challenging. Human fetal multipotent mesenchymal stromal cells (FetMSCs) isolated from early human embryo bone marrow are easy to expand and might be a potential source for pharmaceutical substances production based on their secretome. However, the secretome of FetMSCs was not previously analyzed. Here, we describe the secretome of FetMSCs using LC-MALDI shotgun proteomics. We identified 236 proteins. Functional annotation of the identified proteins revealed their involvement in angiogenesis, ossification, regulation of apoptosis, and immune response processes, which made it promising for biomedical applications. The proteins identified in the FetMSCs secretome are involved in the same biological processes as proteins from previously described adult hMSCs secretomes. Nevertheless, many of the common hMSCs secretome components (such as VEGF, FGF, Wnt and TGF-β) have not been identified in the FetMSCs secretome.
KW - FetMSCs
KW - mesenchymal stem cells
KW - MSCs
KW - multipotent mesenchymal stromal cells
KW - proteomics
KW - regenerative biomedicine
KW - secretome
KW - APOPTOSIS
KW - STEM-CELLS
KW - CURRENT TRENDS
KW - ANGIOGENESIS
KW - PROTECTION
KW - IN-VITRO
KW - REGENERATIVE MEDICINE
KW - DIFFERENTIATION
KW - PROTEINS
KW - LACTADHERIN
UR - http://www.scopus.com/inward/record.url?scp=85096257659&partnerID=8YFLogxK
U2 - 10.3390/molecules25225283
DO - 10.3390/molecules25225283
M3 - Article
C2 - 33198321
AN - SCOPUS:85096257659
VL - 25
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 22
M1 - 5283
ER -
ID: 71413509