TY - JOUR

T1 - Protein glycation and drought response of pea (Pisum sativum L.) root nodule proteome

T2 - a proteomics approach

AU - Shumilina, Julia

AU - Gorbach, Daria

AU - Popova, Veronika

AU - Tsarev, Alexander

AU - Kusnetsova, Alena

AU - Grashina, Maria

AU - Dorn, Mandy

AU - Lukasheva, Elena

AU - Osmolovskaya, Natalia

AU - Romanovskaya, Ekaterina

AU - Zhukov, Vladimir

AU - Ihling, Christian

AU - Grishina, Tatiana

AU - Bilova, Tatiana

AU - Frolov, Andrej

N1 - Publisher Copyright: Copyright: © 2021 Shumilina et al.

PY - 2021/11/12

Y1 - 2021/11/12

N2 - Because of ongoing climate change, drought is becoming the major factor limiting productivity of all plants, including legumes. As these protein-rich crops form symbiotic associations with rhizobial bacteria — root nodules — they readily lose their productivity under drought conditions. Understanding the underlying molecular mechanisms might give access to new strategies to preserve the productivity of legume crops under dehydration. As was shown recently, development of drought response is accompanied by alterations in the patterns of protein glycation and formation of advanced glycation end products (AGEs) that might be a part of unknown regulatory mechanisms. Therefore, here we address the effects of moderate drought on protein dynamics and AGE patterns in pea (Pisum sativum) root nodules. For this, plants inoculated with rhizobial culture were subjected to osmotic stress for one week, harvested, the total protein fraction was isolated from root nodules by phenol extraction, analyzed by bottom-up LC-MS-based proteomics, and AGE patterns were characterized. Surprisingly, despite the clear drought-related changes in phenotype and stomatal conductivity, only minimal accompanying expressional changes (14 rhizobial and 14 pea proteins, mostly involved in central metabolism and nitrogen fixation) could be observed. However, 71 pea and 97 rhizobial proteins (mostly transcription factors, ABC transporters and effector enzymes) were glycated, with carboxymethylation being the major modification type. Thereby, the numbers of glycated sites in nodule proteins dramatically decreased upon stress application. It might indicate an impact of glycation in regulation of transport, protein degradation, central, lipid and nitrogen metabolism. The data are available at Proteome Xchange (accession: PXD024042).

AB - Because of ongoing climate change, drought is becoming the major factor limiting productivity of all plants, including legumes. As these protein-rich crops form symbiotic associations with rhizobial bacteria — root nodules — they readily lose their productivity under drought conditions. Understanding the underlying molecular mechanisms might give access to new strategies to preserve the productivity of legume crops under dehydration. As was shown recently, development of drought response is accompanied by alterations in the patterns of protein glycation and formation of advanced glycation end products (AGEs) that might be a part of unknown regulatory mechanisms. Therefore, here we address the effects of moderate drought on protein dynamics and AGE patterns in pea (Pisum sativum) root nodules. For this, plants inoculated with rhizobial culture were subjected to osmotic stress for one week, harvested, the total protein fraction was isolated from root nodules by phenol extraction, analyzed by bottom-up LC-MS-based proteomics, and AGE patterns were characterized. Surprisingly, despite the clear drought-related changes in phenotype and stomatal conductivity, only minimal accompanying expressional changes (14 rhizobial and 14 pea proteins, mostly involved in central metabolism and nitrogen fixation) could be observed. However, 71 pea and 97 rhizobial proteins (mostly transcription factors, ABC transporters and effector enzymes) were glycated, with carboxymethylation being the major modification type. Thereby, the numbers of glycated sites in nodule proteins dramatically decreased upon stress application. It might indicate an impact of glycation in regulation of transport, protein degradation, central, lipid and nitrogen metabolism. The data are available at Proteome Xchange (accession: PXD024042).

KW - Advanced glycation end products (AGEs)

KW - Drought stress

KW - Glycation

KW - Pea (Pisum sativum)

KW - Polyethylene glycol (PEG)

KW - Proteomics

KW - Root nodules

UR - http://www.scopus.com/inward/record.url?scp=85120435246&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/8d27e659-303f-31fa-afab-d1ad59462bd0/

U2 - 10.21638/SPBU03.2021.303

DO - 10.21638/SPBU03.2021.303

M3 - Article

AN - SCOPUS:85120435246

VL - 66

SP - 210

EP - 214

JO - Biological Communications

JF - Biological Communications

SN - 2542-2154

IS - 3

ER -