Protamine 1 and 2 mRNA Abundance in Human Spermatozoa and Its Relation to Semen Quality and Sperm DNA Fragmentation among Fertility Clinic Patients

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Protamine 1 and 2 mRNA Abundance in Human Spermatozoa and Its Relation to Semen Quality and Sperm DNA Fragmentation among Fertility Clinic Patients. / Ishchuk, M. A.; Malysheva, O. V.; Komarova, E. M.; Mekina, I. D.; Lesik, E. A.; Gzgzyan, A. M.; Kogan, I. Yu ; Baranov, V. S.

In: Russian Journal of Genetics, Vol. 57, No. 2, 02.2021, p. 213-220.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{ca593c6b342a403fac146a6ec474a404,

title = "Protamine 1 and 2 mRNA Abundance in Human Spermatozoa and Its Relation to Semen Quality and Sperm DNA Fragmentation among Fertility Clinic Patients",

abstract = "Abstract: Sperm contain a complex population of coding and noncoding RNA, and the utility of sperm RNA in fertility research is currently being explored. The aim of this study was to estimate the content of transcripts of protamines 1 and 2 in human ejaculated spermatozoa in relation to semen quality and sperm DNA fragmentation between fertile and infertile patients. Human ejaculates were obtained from 33 patients and semen analyzes were assessed by WHO criteria (2010). We evaluated the sperm DNA fragmentation measured by TUNEL assay. The ejaculates of patients were purified by density-gradient centrifugation, sperm cells were lysed, and mRNA was extracted, reverse transcribed, and subjected to real-time qPCR using specific primer pairs for protamine-1, protamine-2 RNA. The sperm protamine mRNA ratio in normozoospermic men (n = 19; 2.86 ± 0.16) differed significantly from that of patozoospermic patients (n = 14; 3.43 ± 0.22; p < 0.05). A significant correlation was shown between sperm DNA fragmentation and the PRM2/PRM1 mRNA ratio (r = 0.33; p < 0.05). In the group of patients with an increased sperm DNA fragmentation (n = 14; 2.74 ± 0.18), the PRM2/PRM1 ratio was significantly higher than in the group of patients with normal rates (n = 19; 3.26 ± 0.19; p < 0.05). An abnormal sperm protamine ratio was associated with poor semen quality and DNA fragmentation. Finding sperm-quality markers would help to understand the causes of male infertility and to improve male reproductive health.",

keywords = "assisted reproductive technologies, human sperm, male infertility, sperm DNA fragmentation, sperm RNA",

author = "Ishchuk, {M. A.} and Malysheva, {O. V.} and Komarova, {E. M.} and Mekina, {I. D.} and Lesik, {E. A.} and Gzgzyan, {A. M.} and Kogan, {I. Yu} and Baranov, {V. S.}",

year = "2021",

month = feb,

doi = "10.1134/s1022795421020058",

language = "English",

volume = "57",

pages = "213--220",

journal = "Russian Journal of Genetics",

issn = "1022-7954",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "2",

}

RIS

TY - JOUR

T1 - Protamine 1 and 2 mRNA Abundance in Human Spermatozoa and Its Relation to Semen Quality and Sperm DNA Fragmentation among Fertility Clinic Patients

AU - Ishchuk, M. A.

AU - Malysheva, O. V.

AU - Komarova, E. M.

AU - Mekina, I. D.

AU - Lesik, E. A.

AU - Gzgzyan, A. M.

AU - Kogan, I. Yu

AU - Baranov, V. S.

PY - 2021/2

Y1 - 2021/2

N2 - Abstract: Sperm contain a complex population of coding and noncoding RNA, and the utility of sperm RNA in fertility research is currently being explored. The aim of this study was to estimate the content of transcripts of protamines 1 and 2 in human ejaculated spermatozoa in relation to semen quality and sperm DNA fragmentation between fertile and infertile patients. Human ejaculates were obtained from 33 patients and semen analyzes were assessed by WHO criteria (2010). We evaluated the sperm DNA fragmentation measured by TUNEL assay. The ejaculates of patients were purified by density-gradient centrifugation, sperm cells were lysed, and mRNA was extracted, reverse transcribed, and subjected to real-time qPCR using specific primer pairs for protamine-1, protamine-2 RNA. The sperm protamine mRNA ratio in normozoospermic men (n = 19; 2.86 ± 0.16) differed significantly from that of patozoospermic patients (n = 14; 3.43 ± 0.22; p < 0.05). A significant correlation was shown between sperm DNA fragmentation and the PRM2/PRM1 mRNA ratio (r = 0.33; p < 0.05). In the group of patients with an increased sperm DNA fragmentation (n = 14; 2.74 ± 0.18), the PRM2/PRM1 ratio was significantly higher than in the group of patients with normal rates (n = 19; 3.26 ± 0.19; p < 0.05). An abnormal sperm protamine ratio was associated with poor semen quality and DNA fragmentation. Finding sperm-quality markers would help to understand the causes of male infertility and to improve male reproductive health.

AB - Abstract: Sperm contain a complex population of coding and noncoding RNA, and the utility of sperm RNA in fertility research is currently being explored. The aim of this study was to estimate the content of transcripts of protamines 1 and 2 in human ejaculated spermatozoa in relation to semen quality and sperm DNA fragmentation between fertile and infertile patients. Human ejaculates were obtained from 33 patients and semen analyzes were assessed by WHO criteria (2010). We evaluated the sperm DNA fragmentation measured by TUNEL assay. The ejaculates of patients were purified by density-gradient centrifugation, sperm cells were lysed, and mRNA was extracted, reverse transcribed, and subjected to real-time qPCR using specific primer pairs for protamine-1, protamine-2 RNA. The sperm protamine mRNA ratio in normozoospermic men (n = 19; 2.86 ± 0.16) differed significantly from that of patozoospermic patients (n = 14; 3.43 ± 0.22; p < 0.05). A significant correlation was shown between sperm DNA fragmentation and the PRM2/PRM1 mRNA ratio (r = 0.33; p < 0.05). In the group of patients with an increased sperm DNA fragmentation (n = 14; 2.74 ± 0.18), the PRM2/PRM1 ratio was significantly higher than in the group of patients with normal rates (n = 19; 3.26 ± 0.19; p < 0.05). An abnormal sperm protamine ratio was associated with poor semen quality and DNA fragmentation. Finding sperm-quality markers would help to understand the causes of male infertility and to improve male reproductive health.

KW - assisted reproductive technologies

KW - human sperm

KW - male infertility

KW - sperm DNA fragmentation

KW - sperm RNA

UR - http://www.scopus.com/inward/record.url?scp=85102931377&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/006c3c33-98cf-3b79-bb24-63af5971338a/

U2 - 10.1134/s1022795421020058

DO - 10.1134/s1022795421020058

M3 - Article

AN - SCOPUS:85102931377

VL - 57

SP - 213

EP - 220

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 2

ER -

ID: 76491460