Research output: Contribution to journal › Article › peer-review
Profiling of non-criteria antiphospholipid antibodies in patients with SLE : differentiation of thrombotic SLE patients and risk of recurrence of thrombosis. / Tkachenko, O; Lapin, S; Mazing, A; Emanuel, V; Belolipetskaia, E; Beliaeva, I; Myachikova, V; Maslyansky, A; Schierack, P; Roggenbuck, D.
In: Lupus, Vol. 29, No. 5, 04.2020, p. 490-498.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Profiling of non-criteria antiphospholipid antibodies in patients with SLE
T2 - differentiation of thrombotic SLE patients and risk of recurrence of thrombosis
AU - Tkachenko, O
AU - Lapin, S
AU - Mazing, A
AU - Emanuel, V
AU - Belolipetskaia, E
AU - Beliaeva, I
AU - Myachikova, V
AU - Maslyansky, A
AU - Schierack, P
AU - Roggenbuck, D
PY - 2020/4
Y1 - 2020/4
N2 - To reveal the clinical significance of criteria and non-criteria antiphospholipid antibodies detected by line immunoassay in comparison with ELISA, systemic lupus erythematosus patients with and without thrombotic events were investigated. Thus, 107 systemic lupus erythematosus patients (48% with deep vein thrombosis or/and arterial thrombosis) and 120 healthy donors were enrolled. Serum antiphospholipid antibodies were detected by ELISA (Orgentec Diagnostika, Germany) and line immunoassay (GA Generic Assays, Germany). Lupus anticoagulant and IgG to cardiolipin and β2GPI but not IgM as well as triple positivity by ELISA and line immunoassay were linked with thrombosis in systemic lupus erythematosus. IgG to phosphatidylinositol and phosphatidylserine by line immunoassay showed significantly higher levels in systemic lupus erythematosus with deep vein thrombosis/arterial thrombosis than without and were independent risk factors for deep vein thrombosis (odds ratio 3.9, 95% confidence interval 1.1, 13.2) and arterial thrombosis (odds ratio 5.1, 95% confidence interval 1.3, 19.8) as well as thrombosis (odds ratio 3.6, 95% confidence interval 1.1, 11.3) and recurrence thereof (odds ratio 6.9, 95% confidence interval 2.1, 22.6), respectively. The occurrence of >4 IgG antiphospholipid antibodies by line immunoassay was an independent risk factor for thrombosis (odds ratio 10.9, 95% confidence interval 1.2, 101.5), arterial thrombosis (odds ratio 14.6, 95% confidence interval 2.5, 86.3), deep vein thrombosis (odds ratio 5.8, 95% confidence interval 1.0, 32.4) and recurrence of thrombosis (odds ratio 35.9, 95% confidence interval 3.8, 342.8). Line immunoassay is a promising multiplex test for the simultaneous detection of criteria and non-criteria antiphospholipid antibodies. Profiling of antiphospholipid antibodies by line immunoassay can differentiate systemic lupus erythematosus patients with thrombosis from systemic lupus erythematosus patients without and assess the risk for thrombosis and recurrence thereof.
AB - To reveal the clinical significance of criteria and non-criteria antiphospholipid antibodies detected by line immunoassay in comparison with ELISA, systemic lupus erythematosus patients with and without thrombotic events were investigated. Thus, 107 systemic lupus erythematosus patients (48% with deep vein thrombosis or/and arterial thrombosis) and 120 healthy donors were enrolled. Serum antiphospholipid antibodies were detected by ELISA (Orgentec Diagnostika, Germany) and line immunoassay (GA Generic Assays, Germany). Lupus anticoagulant and IgG to cardiolipin and β2GPI but not IgM as well as triple positivity by ELISA and line immunoassay were linked with thrombosis in systemic lupus erythematosus. IgG to phosphatidylinositol and phosphatidylserine by line immunoassay showed significantly higher levels in systemic lupus erythematosus with deep vein thrombosis/arterial thrombosis than without and were independent risk factors for deep vein thrombosis (odds ratio 3.9, 95% confidence interval 1.1, 13.2) and arterial thrombosis (odds ratio 5.1, 95% confidence interval 1.3, 19.8) as well as thrombosis (odds ratio 3.6, 95% confidence interval 1.1, 11.3) and recurrence thereof (odds ratio 6.9, 95% confidence interval 2.1, 22.6), respectively. The occurrence of >4 IgG antiphospholipid antibodies by line immunoassay was an independent risk factor for thrombosis (odds ratio 10.9, 95% confidence interval 1.2, 101.5), arterial thrombosis (odds ratio 14.6, 95% confidence interval 2.5, 86.3), deep vein thrombosis (odds ratio 5.8, 95% confidence interval 1.0, 32.4) and recurrence of thrombosis (odds ratio 35.9, 95% confidence interval 3.8, 342.8). Line immunoassay is a promising multiplex test for the simultaneous detection of criteria and non-criteria antiphospholipid antibodies. Profiling of antiphospholipid antibodies by line immunoassay can differentiate systemic lupus erythematosus patients with thrombosis from systemic lupus erythematosus patients without and assess the risk for thrombosis and recurrence thereof.
KW - Adult
KW - Antibodies, Antiphospholipid/blood
KW - Antiphospholipid Syndrome/blood
KW - Case-Control Studies
KW - Female
KW - Humans
KW - Logistic Models
KW - Lupus Coagulation Inhibitor/blood
KW - Lupus Erythematosus, Systemic/blood
KW - Male
KW - Middle Aged
KW - Phosphatidylserines/metabolism
KW - Recurrence
KW - Risk Factors
KW - Venous Thrombosis/complications
U2 - 10.1177/0961203320909952
DO - 10.1177/0961203320909952
M3 - Article
C2 - 32131675
VL - 29
SP - 490
EP - 498
JO - Lupus
JF - Lupus
SN - 0961-2033
IS - 5
ER -
ID: 100706687