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Primary cultures of human colon cancer as a model to study cancer stem cells. / Koshkin, Sergey; Danilova, Anna; Raskin, Grigory; Petrov, Nikolai; Bajenova, Olga; O’Brien, Stephen J.; Tomilin, Alexey; Tolkunova, Elena.

In: Tumor Biology, Vol. 37, No. 9, 01.09.2016, p. 12833-12842.

Research output: Contribution to journal › Article › peer-review

Harvard

Koshkin, S, Danilova, A, Raskin, G, Petrov, N, Bajenova, O, O’Brien, SJ, Tomilin, A & Tolkunova, E 2016, 'Primary cultures of human colon cancer as a model to study cancer stem cells', Tumor Biology, vol. 37, no. 9, pp. 12833-12842. https://doi.org/10.1007/s13277-016-5214-8

APA

Koshkin, S., Danilova, A., Raskin, G., Petrov, N., Bajenova, O., O’Brien, S. J., Tomilin, A., & Tolkunova, E. (2016). Primary cultures of human colon cancer as a model to study cancer stem cells. Tumor Biology, 37(9), 12833-12842. https://doi.org/10.1007/s13277-016-5214-8

Vancouver

Koshkin S, Danilova A, Raskin G, Petrov N, Bajenova O, O’Brien SJ et al. Primary cultures of human colon cancer as a model to study cancer stem cells. Tumor Biology. 2016 Sep 1;37(9):12833-12842. https://doi.org/10.1007/s13277-016-5214-8

Author

Koshkin, Sergey ; Danilova, Anna ; Raskin, Grigory ; Petrov, Nikolai ; Bajenova, Olga ; O’Brien, Stephen J. ; Tomilin, Alexey ; Tolkunova, Elena. / Primary cultures of human colon cancer as a model to study cancer stem cells. In: Tumor Biology. 2016 ; Vol. 37, No. 9. pp. 12833-12842.

BibTeX

@article{0a42e05bc7d64ed6b1597633468aae34,
title = "Primary cultures of human colon cancer as a model to study cancer stem cells",
abstract = "The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion of the primary tumor cells, cancer stem cells (CSCs), which are the most aggressive, have the capacity to metastasize and display properties of stem cells, it is imperative to characterize the gene expression of diagnostic markers and to evaluate the drug sensitivity in the CSCs themselves. Here, we have examined the key genes that are involved in the progression of colorectal cancer and are expressed in cancer stem cells. Primary cultures of colorectal cancer cells from a patient{\textquoteright}s tumors were studied using the flow cytometry and cytological methods. We have evaluated the clinical and stem cell marker expression in these cells, their resistance to 5-fluorouracil and irinotecan, and the ability of cells to form tumors in mice. The data shows the role of stem cell marker Oct4 in the resistance of primary colorectal cancer tumor cells to 5-fluorouracil.",
keywords = "5-Fu, Cancer stem cells, CD133, Colon cancer, Drug resistance, Oct4",
author = "Sergey Koshkin and Anna Danilova and Grigory Raskin and Nikolai Petrov and Olga Bajenova and O{\textquoteright}Brien, {Stephen J.} and Alexey Tomilin and Elena Tolkunova",
year = "2016",
month = sep,
day = "1",
doi = "10.1007/s13277-016-5214-8",
language = "English",
volume = "37",
pages = "12833--12842",
journal = "Tumor Biology",
issn = "1010-4283",
publisher = "Springer Nature",
number = "9",

}

RIS

TY - JOUR

T1 - Primary cultures of human colon cancer as a model to study cancer stem cells

AU - Koshkin, Sergey

AU - Danilova, Anna

AU - Raskin, Grigory

AU - Petrov, Nikolai

AU - Bajenova, Olga

AU - O’Brien, Stephen J.

AU - Tomilin, Alexey

AU - Tolkunova, Elena

PY - 2016/9/1

Y1 - 2016/9/1

N2 - The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion of the primary tumor cells, cancer stem cells (CSCs), which are the most aggressive, have the capacity to metastasize and display properties of stem cells, it is imperative to characterize the gene expression of diagnostic markers and to evaluate the drug sensitivity in the CSCs themselves. Here, we have examined the key genes that are involved in the progression of colorectal cancer and are expressed in cancer stem cells. Primary cultures of colorectal cancer cells from a patient’s tumors were studied using the flow cytometry and cytological methods. We have evaluated the clinical and stem cell marker expression in these cells, their resistance to 5-fluorouracil and irinotecan, and the ability of cells to form tumors in mice. The data shows the role of stem cell marker Oct4 in the resistance of primary colorectal cancer tumor cells to 5-fluorouracil.

AB - The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion of the primary tumor cells, cancer stem cells (CSCs), which are the most aggressive, have the capacity to metastasize and display properties of stem cells, it is imperative to characterize the gene expression of diagnostic markers and to evaluate the drug sensitivity in the CSCs themselves. Here, we have examined the key genes that are involved in the progression of colorectal cancer and are expressed in cancer stem cells. Primary cultures of colorectal cancer cells from a patient’s tumors were studied using the flow cytometry and cytological methods. We have evaluated the clinical and stem cell marker expression in these cells, their resistance to 5-fluorouracil and irinotecan, and the ability of cells to form tumors in mice. The data shows the role of stem cell marker Oct4 in the resistance of primary colorectal cancer tumor cells to 5-fluorouracil.

KW - 5-Fu

KW - Cancer stem cells

KW - CD133

KW - Colon cancer

KW - Drug resistance

KW - Oct4

UR - http://www.scopus.com/inward/record.url?scp=84979272998&partnerID=8YFLogxK

U2 - 10.1007/s13277-016-5214-8

DO - 10.1007/s13277-016-5214-8

M3 - Article

C2 - 27449036

VL - 37

SP - 12833

EP - 12842

JO - Tumor Biology

JF - Tumor Biology

SN - 1010-4283

IS - 9

ER -

ID: 7664375