Polymer particles bearing recombinant lel cd81 as trapping systems for hepatitis c virus

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Polymer particles bearing recombinant lel cd81 as trapping systems for hepatitis c virus. / Polyakov, Dmitry; Sinitsyna, Ekaterina; Grudinina, Natalia; Antipchik, Mariia; Sakhabeev, Rodion; Korzhikov-Vlakh, Viktor; Shavlovsky, Mikhail; Korzhikova-Vlakh, Evgenia ; Tennikova, Tatiana.

In: Pharmaceutics, Vol. 13, No. 5, 672, 07.05.2021.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{4ac2a644a6934c3399b8d3727be72bba,

title = "Polymer particles bearing recombinant lel cd81 as trapping systems for hepatitis c virus",

abstract = "Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro-and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro-and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro-and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods.",

keywords = "Interaction of LEL CD81 with E2 protein, Poly(lactic acid)-based micro-and nanoparticles, Recombinant CD81-streptavidin fusion protein, Trapping system for HCV, Virus-mimicking particles, recombinant CD81-streptavidin fusion protein, SIZE, NANOTRAP, ENVELOPE PROTEINS, IDENTIFICATION, E2, virus-mimicking particles, NANOPARTICLES, trapping system for HCV, poly(lactic acid)-based micro- and nanoparticles, PURIFICATION, interaction of LEL CD81 with E2 protein, ALBUMIN, MICRO, EXPRESSION",

author = "Dmitry Polyakov and Ekaterina Sinitsyna and Natalia Grudinina and Mariia Antipchik and Rodion Sakhabeev and Viktor Korzhikov-Vlakh and Mikhail Shavlovsky and Evgenia Korzhikova-Vlakh and Tatiana Tennikova",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = may,

day = "7",

doi = "10.3390/pharmaceutics13050672",

language = "English",

volume = "13",

journal = "Pharmaceutics",

issn = "1999-4923",

publisher = "MDPI AG",

number = "5",

}

RIS

TY - JOUR

T1 - Polymer particles bearing recombinant lel cd81 as trapping systems for hepatitis c virus

AU - Polyakov, Dmitry

AU - Sinitsyna, Ekaterina

AU - Grudinina, Natalia

AU - Antipchik, Mariia

AU - Sakhabeev, Rodion

AU - Korzhikov-Vlakh, Viktor

AU - Shavlovsky, Mikhail

AU - Korzhikova-Vlakh, Evgenia

AU - Tennikova, Tatiana

PY - 2021/5/7

Y1 - 2021/5/7

N2 - Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro-and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro-and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro-and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods.

AB - Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro-and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro-and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro-and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods.

KW - Interaction of LEL CD81 with E2 protein

KW - Poly(lactic acid)-based micro-and nanoparticles

KW - Recombinant CD81-streptavidin fusion protein

KW - Trapping system for HCV

KW - Virus-mimicking particles

KW - recombinant CD81-streptavidin fusion protein

KW - SIZE

KW - NANOTRAP

KW - ENVELOPE PROTEINS

KW - IDENTIFICATION

KW - E2

KW - virus-mimicking particles

KW - NANOPARTICLES

KW - trapping system for HCV

KW - poly(lactic acid)-based micro- and nanoparticles

KW - PURIFICATION

KW - interaction of LEL CD81 with E2 protein

KW - ALBUMIN

KW - MICRO

KW - EXPRESSION

UR - http://www.scopus.com/inward/record.url?scp=85106472030&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/3758d17d-e291-39c2-8f40-b16acaabc5c8/

U2 - 10.3390/pharmaceutics13050672

DO - 10.3390/pharmaceutics13050672

M3 - Article

AN - SCOPUS:85106472030

VL - 13

JO - Pharmaceutics

JF - Pharmaceutics

SN - 1999-4923

IS - 5

M1 - 672

ER -

ID: 83919888