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Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs. / Korzhikov-Vlakh, Viktor ; Katernuk, Iuliia ; Pilipenko, Iuliia ; Lavrentieva, Antonina ; Guryanov, Ivan ; Sharoyko, Vladimir ; Manshina, Alina A. ; Tennikova, Tatiana B. .

In: Polymers, Vol. 12, No. 5, 1077, 01.05.2020.

Research output: Contribution to journal › Article › peer-review

Harvard

Korzhikov-Vlakh, V, Katernuk, I, Pilipenko, I, Lavrentieva, A, Guryanov, I, Sharoyko, V, Manshina, AA & Tennikova, TB 2020, 'Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs', Polymers, vol. 12, no. 5, 1077. https://doi.org/10.3390/POLYM12051077

APA

Korzhikov-Vlakh, V., Katernuk, I., Pilipenko, I., Lavrentieva, A., Guryanov, I., Sharoyko, V., Manshina, A. A., & Tennikova, T. B. (2020). Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs. Polymers, 12(5), [1077]. https://doi.org/10.3390/POLYM12051077

Vancouver

Korzhikov-Vlakh V, Katernuk I, Pilipenko I, Lavrentieva A, Guryanov I, Sharoyko V et al. Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs. Polymers. 2020 May 1;12(5). 1077. https://doi.org/10.3390/POLYM12051077

Author

Korzhikov-Vlakh, Viktor ; Katernuk, Iuliia ; Pilipenko, Iuliia ; Lavrentieva, Antonina ; Guryanov, Ivan ; Sharoyko, Vladimir ; Manshina, Alina A. ; Tennikova, Tatiana B. . / Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs. In: Polymers. 2020 ; Vol. 12, No. 5.

BibTeX

@article{75d2ae45fa644e9a84c2a255848e0052,
title = "Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs",
abstract = "Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model oligonucleotide, siRNA, and pDNA—were obtained. Poly-l-lysineto heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics ofphotosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.",
keywords = "poly-l-lysine, heparin, interpolyelectrolyte complex, photosensitive, siRNA, pDNA, PDNA, Photosensitive, Heparin, Interpolyelectrolyte complex, SiRNA, Poly-l-lysine",
author = "Viktor Korzhikov-Vlakh and Iuliia Katernuk and Iuliia Pilipenko and Antonina Lavrentieva and Ivan Guryanov and Vladimir Sharoyko and Manshina, {Alina A.} and Tennikova, {Tatiana B.}",
note = "Publisher Copyright: {\textcopyright} 2020 by the authors.",
year = "2020",
month = may,
day = "1",
doi = "10.3390/POLYM12051077",
language = "English",
volume = "12",
journal = "Polymers",
issn = "2073-4360",
publisher = "MDPI AG",
number = "5",

}

RIS

TY - JOUR

T1 - Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs

AU - Korzhikov-Vlakh, Viktor

AU - Katernuk, Iuliia

AU - Pilipenko, Iuliia

AU - Lavrentieva, Antonina

AU - Guryanov, Ivan

AU - Sharoyko, Vladimir

AU - Manshina, Alina A.

AU - Tennikova, Tatiana B.

N1 - Publisher Copyright: © 2020 by the authors.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model oligonucleotide, siRNA, and pDNA—were obtained. Poly-l-lysineto heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics ofphotosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.

AB - Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model oligonucleotide, siRNA, and pDNA—were obtained. Poly-l-lysineto heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics ofphotosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.

KW - poly-l-lysine

KW - heparin

KW - interpolyelectrolyte complex

KW - photosensitive

KW - siRNA

KW - pDNA

KW - PDNA

KW - Photosensitive

KW - Heparin

KW - Interpolyelectrolyte complex

KW - SiRNA

KW - Poly-l-lysine

UR - https://www.mdpi.com/2073-4360/12/5/1077

UR - http://www.scopus.com/inward/record.url?scp=85085328279&partnerID=8YFLogxK

U2 - 10.3390/POLYM12051077

DO - 10.3390/POLYM12051077

M3 - Article

VL - 12

JO - Polymers

JF - Polymers

SN - 2073-4360

IS - 5

M1 - 1077

ER -

ID: 53596602