TY - JOUR

T1 - Pheromone of grouped female mice impairs genome stability in male mice through stress-mediated pathways

AU - Глинин, Тимофей Сергеевич

AU - Петрова, Марина Викторовна

AU - Щербинина, Вероника Дмитриевна

AU - Шубина, Анастасия Николаевна

AU - Дукельская, Анна Владимировна

AU - Старшова, Полина Андреевна

AU - Мамонтова, Виктория Андреевна

AU - Бурнусуз, Александра Валентиновна

AU - Ромащенко, Александр

AU - Мошкин, Михаил

AU - Хайтович, Филипп

AU - Даев, Евгений Владиславович

PY - 2023/10/17

Y1 - 2023/10/17

N2 - Population density is known to affect the health and survival of many species, and is especially important for social animals. In mice, living in crowded conditions results in the disruption of social interactions, chronic stress, and immune and reproductive suppression; however, the underlying mechanisms remain unclear. Here, we investigated the role of chemosignals in the regulation of mouse physiology and behavior in response to social crowding. The pheromone 2,5-dimethylpyrazine (2,5-DMP), which is released by female mice in crowded conditions, induced aversion, glucocorticoid elevation and, when chronic, resulted in reproductive and immune suppression. 2,5-DMP olfaction induced genome destabilization in bone marrow cells in a stress-dependent manner, providing a plausible mechanism for crowding-induced immune dysfunction. Interestingly, the genome-destabilizing effect of 2,5-DMP was comparable to a potent mouse stressor (immobilization), and both stressors led to correlated expression changes in genes regulating cellular stress response. Thus, our findings demonstrate that, in mice, the health effects of crowding may be explained at least in part by chemosignals and also propose a significant role of stress and genome destabilization in the emergence of crowding effects.

AB - Population density is known to affect the health and survival of many species, and is especially important for social animals. In mice, living in crowded conditions results in the disruption of social interactions, chronic stress, and immune and reproductive suppression; however, the underlying mechanisms remain unclear. Here, we investigated the role of chemosignals in the regulation of mouse physiology and behavior in response to social crowding. The pheromone 2,5-dimethylpyrazine (2,5-DMP), which is released by female mice in crowded conditions, induced aversion, glucocorticoid elevation and, when chronic, resulted in reproductive and immune suppression. 2,5-DMP olfaction induced genome destabilization in bone marrow cells in a stress-dependent manner, providing a plausible mechanism for crowding-induced immune dysfunction. Interestingly, the genome-destabilizing effect of 2,5-DMP was comparable to a potent mouse stressor (immobilization), and both stressors led to correlated expression changes in genes regulating cellular stress response. Thus, our findings demonstrate that, in mice, the health effects of crowding may be explained at least in part by chemosignals and also propose a significant role of stress and genome destabilization in the emergence of crowding effects.

UR - https://www.mendeley.com/catalogue/07025859-75c8-38fe-85ec-d224913fb2be/

U2 - 10.1038/s41598-023-44647-w

DO - 10.1038/s41598-023-44647-w

M3 - Article

C2 - 37848549

VL - 13

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 17622

ER -