TY - JOUR

T1 - Overexpression of isoform B of Dgp-1 gene enhances locomotor activity in senescent Drosophila males and under heat stress

AU - Fedotov, Sergey A.

AU - Besedina, Natalia G.

AU - Bragina, Julia V.

AU - Danilenkova, Larisa V.

AU - Kamysheva, Elena A.

AU - Kamyshev, Nikolai G.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Here, we describe the longevity and locomotor behavior of senescent Drosophila males with altered expression of Dgp-1 gene. In comparison with the wild-type Canton-S (CS) males, six characteristics of the phenotype of Dgp-1[843k] mutant were found: (1) low expression of isoform A; (2) augmented expression of isoform B; (3) reduction in the mean lifespan; (4) decrease in the running speed in 3-day-old flies; (5) maintenance of a high run frequency in senescent flies; and (6) resistance to heat stress manifested as maintenance of a high run frequency at 29 °C. After cessation of “cantonization” process, mean lifespan of the mutant males drifted from low to high values finally exceeding that for CS. In contrast, behavioral phenotype of the mutant was robust. Using the GAL4/UAS system, we showed that neurospecific overexpression of isoform B resulted in a slight decrease of longevity and a high level of run frequency in the senescent flies, similar to that in Dgp-1[843K] mutant. In addition, a decreased level of reactive oxygen species was found in Dgp-1[843K] mutant males maintained under stress conditions. The elevated resistance to oxidative stress probably explains the two distinctive features of the mutation: resistance to aging processes and thermal stress displayed at behavioral level.

AB - Here, we describe the longevity and locomotor behavior of senescent Drosophila males with altered expression of Dgp-1 gene. In comparison with the wild-type Canton-S (CS) males, six characteristics of the phenotype of Dgp-1[843k] mutant were found: (1) low expression of isoform A; (2) augmented expression of isoform B; (3) reduction in the mean lifespan; (4) decrease in the running speed in 3-day-old flies; (5) maintenance of a high run frequency in senescent flies; and (6) resistance to heat stress manifested as maintenance of a high run frequency at 29 °C. After cessation of “cantonization” process, mean lifespan of the mutant males drifted from low to high values finally exceeding that for CS. In contrast, behavioral phenotype of the mutant was robust. Using the GAL4/UAS system, we showed that neurospecific overexpression of isoform B resulted in a slight decrease of longevity and a high level of run frequency in the senescent flies, similar to that in Dgp-1[843K] mutant. In addition, a decreased level of reactive oxygen species was found in Dgp-1[843K] mutant males maintained under stress conditions. The elevated resistance to oxidative stress probably explains the two distinctive features of the mutation: resistance to aging processes and thermal stress displayed at behavioral level.

KW - Drosophila Dgp-1 mutant

KW - Heat stress

KW - Locomotion

KW - Oxidative stress

KW - Senescence

KW - OXIDATIVE STRESS

KW - SLEEP

KW - PARKIN

KW - RESPONSES

KW - AGE-RELATED IMPAIRMENT

KW - MUTATIONS

KW - MELANOGASTER

KW - EXPRESSION

KW - TOOL

UR - http://www.scopus.com/inward/record.url?scp=85074815871&partnerID=8YFLogxK

U2 - 10.1007/s00359-019-01378-6

DO - 10.1007/s00359-019-01378-6

M3 - Article

AN - SCOPUS:85074815871

VL - 205

SP - 897

EP - 910

JO - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology

JF - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology

SN - 0340-7594

IS - 6

ER -