TY - JOUR

T1 - Optimization of the Conditions of Analysis of Exhaled Air by Gas Chromatography–Mass Spectrometry for the Noninvasive Diagnostics of Lung Cancer

AU - Gorbunov, I. S.

AU - Gubal’, A. R.

AU - Ganeev, A. A.

AU - Rodinkov, O. V.

AU - Kartsova, L. A.

AU - Bessonova, E. A.

AU - Arsen’ev, A. I.

AU - Nefedov, A. O.

AU - Kraeva, L. A.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - We optimized procedures for the collection, preparation, and storage of samples of exhaled air and the conditions for determining volatile organic compounds in exhaled air by gas chromatography-mass spectrometry (GC-MS), the presence or change in the concentration of which can be caused by lung cancer. The analysis circuit includes group adsorption preconcentration followed by the thermal desorption and determination of analytes by GC-MS. Polymer bags of a Tedlar film were used for sample collection. The conditions and periods of the storage of samples in bags and adsorption tubes are determined. A method for cleaning and conditioning bags is proposed. For preconcentrating exhaled air components, we used hydrophobic adsorbents (Porapak (TM) P, Porapak (TM) Q, and Tenax TA) and three-section tubes containing Tenax GR, Carbopack (TM) B, and Carbosieve S-III. Their relative efficiency was evaluated by assessing the residual background of gas emission from the adsorbent, the magnitude of the analytical signal, the efficiency of adsorption/desorption, and the efficiency of storage. The conditions for the preconcentration of volatile organic compounds (volume and rate of aspiration, time, and temperature of thermal desorption) are optimized. The main volatile organic compounds in the exhaled air of conditionally healthy volunteers were identified, and their concentrations are evaluated.

AB - We optimized procedures for the collection, preparation, and storage of samples of exhaled air and the conditions for determining volatile organic compounds in exhaled air by gas chromatography-mass spectrometry (GC-MS), the presence or change in the concentration of which can be caused by lung cancer. The analysis circuit includes group adsorption preconcentration followed by the thermal desorption and determination of analytes by GC-MS. Polymer bags of a Tedlar film were used for sample collection. The conditions and periods of the storage of samples in bags and adsorption tubes are determined. A method for cleaning and conditioning bags is proposed. For preconcentrating exhaled air components, we used hydrophobic adsorbents (Porapak (TM) P, Porapak (TM) Q, and Tenax TA) and three-section tubes containing Tenax GR, Carbopack (TM) B, and Carbosieve S-III. Their relative efficiency was evaluated by assessing the residual background of gas emission from the adsorbent, the magnitude of the analytical signal, the efficiency of adsorption/desorption, and the efficiency of storage. The conditions for the preconcentration of volatile organic compounds (volume and rate of aspiration, time, and temperature of thermal desorption) are optimized. The main volatile organic compounds in the exhaled air of conditionally healthy volunteers were identified, and their concentrations are evaluated.

KW - adsorption preconcentration

KW - exhaled air

KW - gas chromatography–mass spectrometry

KW - lung cancer

KW - volatile organic compounds

KW - BIOMARKERS

KW - BREATH ANALYSIS

KW - gas chromatography-mass spectrometry

KW - SOLID-PHASE MICROEXTRACTION

KW - MARKERS

KW - VOLATILE ORGANIC-COMPOUNDS

UR - http://www.scopus.com/inward/record.url?scp=85074249407&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/optimization-conditions-analysis-exhaled-air-gas-chromatographymass-spectrometry-noninvasive-diagnos

U2 - 10.1134/S1061934819110042

DO - 10.1134/S1061934819110042

M3 - Article

AN - SCOPUS:85074249407

VL - 74

SP - 1148

EP - 1158

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 11

ER -