TY - JOUR

T1 - Opine biosynthesis in naturally transgenic plants

T2 - Genes and products

AU - Matveeva, Tatiana

AU - Otten, Léon

N1 - Publisher Copyright: © 2021 Elsevier Ltd

PY - 2021/9/1

Y1 - 2021/9/1

N2 - The plant pathogen Agrobacterium transfers DNA into plant cells by a specific transfer mechanism. Expression of this transferred DNA or T-DNA leads to crown gall tumors or abnormal, hairy roots and the synthesis of specific compounds, called opines. Opines are produced from common plant metabolites like sugars, amino acids and α-keto acids, which are combined into different low molecular weight structures by T-DNA-encoded opine synthase enzymes. Opines can be converted back by Agrobacterium into the original metabolites and used for agrobacterial growth. Recently it has been discovered that about 7% of Angiosperms carry T-DNA-like sequences. These result from ancient Agrobacterium transformation events, followed by spontaneous regeneration of transformed cells into natural genetically transformed organisms (nGMOs). Nearly all nGMOs identified up to date carry opine synthesis genes, several of these are intact and potentially encode opine synthesis. So far, only tobacco and cuscuta have been demonstrated to contain opines. Whereas opines from crown gall and hairy root tissues have been studied for over 60 years, those from the nGMOs remain to be explored.

AB - The plant pathogen Agrobacterium transfers DNA into plant cells by a specific transfer mechanism. Expression of this transferred DNA or T-DNA leads to crown gall tumors or abnormal, hairy roots and the synthesis of specific compounds, called opines. Opines are produced from common plant metabolites like sugars, amino acids and α-keto acids, which are combined into different low molecular weight structures by T-DNA-encoded opine synthase enzymes. Opines can be converted back by Agrobacterium into the original metabolites and used for agrobacterial growth. Recently it has been discovered that about 7% of Angiosperms carry T-DNA-like sequences. These result from ancient Agrobacterium transformation events, followed by spontaneous regeneration of transformed cells into natural genetically transformed organisms (nGMOs). Nearly all nGMOs identified up to date carry opine synthesis genes, several of these are intact and potentially encode opine synthesis. So far, only tobacco and cuscuta have been demonstrated to contain opines. Whereas opines from crown gall and hairy root tissues have been studied for over 60 years, those from the nGMOs remain to be explored.

KW - Horizontal gene transfer

KW - Natural genetically modified organisms

KW - Opine synthases

KW - Opines

KW - Rhizobium rhizogenes (Riker et al., 1930) Young et al., 2001 (Rhizobiaceae)

KW - T-DNAs

KW - Tobacco

KW - Plant Tumors

KW - Amino Acids

KW - Plants, Genetically Modified/genetics

KW - CROWN-GALL TUMORS

KW - Opines Opine synthases

KW - RHIZOGENES TL-DNA

KW - 2001 (Rhizobiaceae)

KW - Rhizobium rhizogenes (Riker et al., 1930) Young et al.

KW - ROL GENES

KW - TI-PLASMID

KW - HAIRY ROOT

KW - AGROBACTERIUM-TUMEFACIENS

KW - CATABOLIC MANNOPINE CYCLASE

KW - TOBACCO PLANTS

KW - AGROCINOPINE-A

KW - OCTOPINE DEHYDROGENASE

UR - http://www.scopus.com/inward/record.url?scp=85111061011&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/d61c8df4-c294-3c1d-8470-3d2ec7312abf/

U2 - 10.1016/j.phytochem.2021.112813

DO - 10.1016/j.phytochem.2021.112813

M3 - Article

C2 - 34192603

AN - SCOPUS:85111061011

VL - 189

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

M1 - 112813

ER -