Research output: Contribution to journal › Article
Opine biosynthesis in naturally transgenic plants : Genes and products. / Matveeva, Tatiana; Otten, Léon.
In: Phytochemistry, Vol. 189, 112813, 01.09.2021.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Opine biosynthesis in naturally transgenic plants
T2 - Genes and products
AU - Matveeva, Tatiana
AU - Otten, Léon
N1 - Publisher Copyright: © 2021 Elsevier Ltd
PY - 2021/9/1
Y1 - 2021/9/1
N2 - The plant pathogen Agrobacterium transfers DNA into plant cells by a specific transfer mechanism. Expression of this transferred DNA or T-DNA leads to crown gall tumors or abnormal, hairy roots and the synthesis of specific compounds, called opines. Opines are produced from common plant metabolites like sugars, amino acids and α-keto acids, which are combined into different low molecular weight structures by T-DNA-encoded opine synthase enzymes. Opines can be converted back by Agrobacterium into the original metabolites and used for agrobacterial growth. Recently it has been discovered that about 7% of Angiosperms carry T-DNA-like sequences. These result from ancient Agrobacterium transformation events, followed by spontaneous regeneration of transformed cells into natural genetically transformed organisms (nGMOs). Nearly all nGMOs identified up to date carry opine synthesis genes, several of these are intact and potentially encode opine synthesis. So far, only tobacco and cuscuta have been demonstrated to contain opines. Whereas opines from crown gall and hairy root tissues have been studied for over 60 years, those from the nGMOs remain to be explored.
AB - The plant pathogen Agrobacterium transfers DNA into plant cells by a specific transfer mechanism. Expression of this transferred DNA or T-DNA leads to crown gall tumors or abnormal, hairy roots and the synthesis of specific compounds, called opines. Opines are produced from common plant metabolites like sugars, amino acids and α-keto acids, which are combined into different low molecular weight structures by T-DNA-encoded opine synthase enzymes. Opines can be converted back by Agrobacterium into the original metabolites and used for agrobacterial growth. Recently it has been discovered that about 7% of Angiosperms carry T-DNA-like sequences. These result from ancient Agrobacterium transformation events, followed by spontaneous regeneration of transformed cells into natural genetically transformed organisms (nGMOs). Nearly all nGMOs identified up to date carry opine synthesis genes, several of these are intact and potentially encode opine synthesis. So far, only tobacco and cuscuta have been demonstrated to contain opines. Whereas opines from crown gall and hairy root tissues have been studied for over 60 years, those from the nGMOs remain to be explored.
KW - Horizontal gene transfer
KW - Natural genetically modified organisms
KW - Opine synthases
KW - Opines
KW - Rhizobium rhizogenes (Riker et al., 1930) Young et al., 2001 (Rhizobiaceae)
KW - T-DNAs
KW - Tobacco
KW - Plant Tumors
KW - Amino Acids
KW - Plants, Genetically Modified/genetics
KW - CROWN-GALL TUMORS
KW - Opines Opine synthases
KW - RHIZOGENES TL-DNA
KW - 2001 (Rhizobiaceae)
KW - Rhizobium rhizogenes (Riker et al., 1930) Young et al.
KW - ROL GENES
KW - TI-PLASMID
KW - HAIRY ROOT
KW - AGROBACTERIUM-TUMEFACIENS
KW - CATABOLIC MANNOPINE CYCLASE
KW - TOBACCO PLANTS
KW - AGROCINOPINE-A
KW - OCTOPINE DEHYDROGENASE
UR - http://www.scopus.com/inward/record.url?scp=85111061011&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/d61c8df4-c294-3c1d-8470-3d2ec7312abf/
U2 - 10.1016/j.phytochem.2021.112813
DO - 10.1016/j.phytochem.2021.112813
M3 - Article
C2 - 34192603
AN - SCOPUS:85111061011
VL - 189
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
M1 - 112813
ER -
ID: 85150444