Efficient engineering of silver nanofibers is of great importance for construction of various functional devices from optoelectronics to sensing technologies. This study presents facile one-step synthesis of crystalline silver nanofibers directly on a substrate surface. The fabrication process is irradiation of the substrate/liquid interface with low-intensity laser irradiation. As a liquid, water solution of silver bensoate hydrate is used without any additional reducing, capping agents, catalyzers, or surfactants. Widely available continuous wave lasers of broad spectral region (266, 374, 405, 448 nm) and illumination time for 30 min were found to be efficient for the fabrication of Ag nanofibers coatings. Thus laser-induced deposition provided fabrication of long-winding nanofibers consisting of well-formed Ag nanocrystals with diameter ca 60–80 nm. Coatings of Ag nanofibers are formed in the laser-affected area of the substrate. The stages of the process are formation of branched organic structures from the benzoic anion followed by nucleation and growth of silver nanocrystals thus providing self-template approach not requiring additional stages of components pre-synthesis. Thus laser-induced deposition from water solution of ecologically friendly precursor allows fabrication of Ag nanofibers directly on a substrate surface which could be a promising and easily scalable approach for wide production.