Research output: Contribution to journal › Article › peer-review
One-Pot Synthesis of Silica-Coated Gold Nanostructures Loaded with Cyanine 5.5 for Cell Imaging by SERS Spectroscopy. / Smirnov, Aleksei N. ; Aslanov, Simar F. ; Danilov, Denis V. ; Kurapova, Olga Yu. ; Solovyeva, Elena V. .
In: Nanomaterials, Vol. 13, No. 7, 1267, 03.04.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - One-Pot Synthesis of Silica-Coated Gold Nanostructures Loaded with Cyanine 5.5 for Cell Imaging by SERS Spectroscopy
AU - Smirnov, Aleksei N.
AU - Aslanov, Simar F.
AU - Danilov, Denis V.
AU - Kurapova, Olga Yu.
AU - Solovyeva, Elena V.
N1 - : Smirnov, A.N.; Aslanov, S.F.; Danilov, D.V.; Kurapova, O.Y.; Solovyeva, E.V. One-Pot Synthesis of Silica-Coated Gold Nanostructures Loaded with Cyanine 5.5 for Cell Imaging by SERS Spectroscopy. Nanomaterials 2023, 13, 1267. https://doi.org/10.3390/nano13071267
PY - 2023/4/3
Y1 - 2023/4/3
N2 - Anisotropic gold nanoparticles have been recognized as promising agents for medicaldiagnostics and cancer therapy due to their wide functionality, photothermal effect, and ability foroptical signal amplification in the near-infrared range. In this work, a simple and rapid method forthe preparation of bone-shaped gold nanoparticles coated with a dye-impregnated silica shell withan aminated surface is proposed. The possibility of further functionalization the nanostructures witha delivery vector using folic acid as an example is demonstrated. The average size of the resultingtags does not exceed 70 nm, meeting the criteria of cell endocytosis. The prepared tags exhibitsurface-enhanced Raman scattering (SERS) spectra at excitation with lasers of 632.8 and 785 nm. Cellimaging is performed on HeLa cells based on the most pronounced SERS bands as a tracking signal.The obtained images, along with scanning electron microscopy of cell samples, revealed the tendencyof tags to agglomerate during endocytosis followed by the “hot spots” effect. To evaluate the toxicand proliferative effect of the nanotags, an MTT assay was performed with two HeLa and HEP G2cell lines. The results revealed higher viability for HEP G2 cells.
AB - Anisotropic gold nanoparticles have been recognized as promising agents for medicaldiagnostics and cancer therapy due to their wide functionality, photothermal effect, and ability foroptical signal amplification in the near-infrared range. In this work, a simple and rapid method forthe preparation of bone-shaped gold nanoparticles coated with a dye-impregnated silica shell withan aminated surface is proposed. The possibility of further functionalization the nanostructures witha delivery vector using folic acid as an example is demonstrated. The average size of the resultingtags does not exceed 70 nm, meeting the criteria of cell endocytosis. The prepared tags exhibitsurface-enhanced Raman scattering (SERS) spectra at excitation with lasers of 632.8 and 785 nm. Cellimaging is performed on HeLa cells based on the most pronounced SERS bands as a tracking signal.The obtained images, along with scanning electron microscopy of cell samples, revealed the tendencyof tags to agglomerate during endocytosis followed by the “hot spots” effect. To evaluate the toxicand proliferative effect of the nanotags, an MTT assay was performed with two HeLa and HEP G2cell lines. The results revealed higher viability for HEP G2 cells.
KW - gold nanoparticles
KW - silicon oxide
KW - one-pot synthesis
KW - tag
KW - SERS
KW - bioimaging
KW - cyanine
KW - gold nanoparticles
KW - silicon oxide
KW - one-pot synthesis
KW - tag
KW - SERS
KW - bioimaging
KW - cyanine
UR - https://www.mdpi.com/2079-4991/13/7/1267
M3 - Article
VL - 13
JO - Nanomaterials
JF - Nanomaterials
SN - 2079-4991
IS - 7
M1 - 1267
ER -
ID: 104393925