Research output: Contribution to journal › Article › peer-review
On the phylogenetic position of Raphidocystis pallida with some notes on its life cycle. / Drachko, Daria; Shɨshkin, Yegor; Zlatogursky, Vasily V.
In: Journal of Eukaryotic Microbiology, Vol. 69, No. 4, e12916, 01.07.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - On the phylogenetic position of Raphidocystis pallida with some notes on its life cycle
AU - Drachko, Daria
AU - Shɨshkin, Yegor
AU - Zlatogursky, Vasily V.
N1 - Publisher Copyright: © 2022 International Society of Protistologists.
PY - 2022/7/1
Y1 - 2022/7/1
N2 - Raphidocystis pallida, a centrohelid heliozoan with unusually shaped tubular siliceous scales, was reisolated from Jamor river, Portugal, and studied with the use of light and electron microscopy. In enriched cultures, the cells were naked, devoid of siliceous external skeleton with the exception of several scales present in one cell. Instead, such cells were covered with a layer of rod-shaped bacteria. In clonal cultures, the cells gradually acquired siliceous coverings typical for this species and retained them in next generations. Phylogenetic position of R. pallida was clarified with SSU rDNA-based molecular phylogenetics, and its placement within the genus Raphidocystis despite unusual coverings structure was confirmed. The implications of phylogenetic placement of R. pallida and possible origins of the previously undescribed naked form were discussed.
AB - Raphidocystis pallida, a centrohelid heliozoan with unusually shaped tubular siliceous scales, was reisolated from Jamor river, Portugal, and studied with the use of light and electron microscopy. In enriched cultures, the cells were naked, devoid of siliceous external skeleton with the exception of several scales present in one cell. Instead, such cells were covered with a layer of rod-shaped bacteria. In clonal cultures, the cells gradually acquired siliceous coverings typical for this species and retained them in next generations. Phylogenetic position of R. pallida was clarified with SSU rDNA-based molecular phylogenetics, and its placement within the genus Raphidocystis despite unusual coverings structure was confirmed. The implications of phylogenetic placement of R. pallida and possible origins of the previously undescribed naked form were discussed.
KW - centrohelids
KW - external skeleton
KW - heliozoa
KW - life cycles
KW - systematics
KW - ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=85129648808&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/c21cc0cb-44c6-317f-8361-d6b5db861c6f/
U2 - 10.1111/jeu.12916
DO - 10.1111/jeu.12916
M3 - Article
AN - SCOPUS:85129648808
VL - 69
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
SN - 1066-5234
IS - 4
M1 - e12916
ER -
ID: 96556254