TY - JOUR

T1 - On particular Regimens of Derivative UV Spectrophotometry for Comparative Analysis of Proteins

AU - Lianguzov, A. Y.

AU - Malygina, N. M.

AU - Ivanov, A. M.

AU - Petrova, T. A.

PY - 2022/12

Y1 - 2022/12

N2 - Bovine serum albumin and two oxygen transport proteins, hemocyanin from the snail Achatinafulica and bovine hemoglobin, were used to define what regimens of derivative UV spectrophotometry aremost appropriate for using it as an express technique for nondestructive comparative analysis of native proteins preparations. It was found that the fourth derivatives of protein absorption spectra make it possible todetect the individual bands of aromatic amino acids in a way that is optimal for solving certain practical problems. An algorithm for calculating the fourth derivatives was selected experimentally. To verify the approach,the fourth derivatives of the native proteins spectra were reconstructed by combining those of individual aromatic amino-acid spectra in the range of 240–300 nm. To demonstrate the individual differences betweenproteins, it is proposed to use the correlation coefficients of the fourth derivatives of spectra in the range of240–300 nm or in the wavelength range of tyrosine and tryptophan. Although this approach does not allowestimation of the exact contents of individual aromatic amino acids in proteins, it allows comparing differentproteins between each other. The proposed approach makes it possible to obtain an individual spectral “portrait” of a protein, which distinguishes it from other proteins and is useful as a reference for further experimental work with it.

AB - Bovine serum albumin and two oxygen transport proteins, hemocyanin from the snail Achatinafulica and bovine hemoglobin, were used to define what regimens of derivative UV spectrophotometry aremost appropriate for using it as an express technique for nondestructive comparative analysis of native proteins preparations. It was found that the fourth derivatives of protein absorption spectra make it possible todetect the individual bands of aromatic amino acids in a way that is optimal for solving certain practical problems. An algorithm for calculating the fourth derivatives was selected experimentally. To verify the approach,the fourth derivatives of the native proteins spectra were reconstructed by combining those of individual aromatic amino-acid spectra in the range of 240–300 nm. To demonstrate the individual differences betweenproteins, it is proposed to use the correlation coefficients of the fourth derivatives of spectra in the range of240–300 nm or in the wavelength range of tyrosine and tryptophan. Although this approach does not allowestimation of the exact contents of individual aromatic amino acids in proteins, it allows comparing differentproteins between each other. The proposed approach makes it possible to obtain an individual spectral “portrait” of a protein, which distinguishes it from other proteins and is useful as a reference for further experimental work with it.

KW - Cпектры поглощения белков, производная УФ-спектрофотометрия, бычий сывороточный альбумин, гемоглобин, гемоцианин, фенилаланин, тирозин, триптофан, сравнительный анализ белков

KW - proteins absorption spectra

KW - derivative UV spectrophotometry

KW - bovine serum albumin

KW - hemoglobin

KW - hemocyanin

KW - phenylalanine

KW - tyrosine

KW - tryptophan

KW - proteins comparative analysis

M3 - Article

VL - 58

SP - S153–S159

JO - Applied Biochemistry and Microbiology

JF - Applied Biochemistry and Microbiology

SN - 0003-6838

IS - Suppl. 1

ER -