Research output: Contribution to conference › Abstract
Obtaining and the specificity characterization of antibodies against the plant signaling peptide CLE41/44 by gold nanoparticle conjugates. / Burygin, G.L.; Dykman, L.A.; Lutova, L.A.
2020. 1145716 Abstract fromResearch output: Contribution to conference › Abstract
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TY - CONF
T1 - Obtaining and the specificity characterization of antibodies against the plant signaling peptide CLE41/44 by gold nanoparticle conjugates.
AU - Burygin, G.L.
AU - Dykman, L.A.
AU - Lutova, L.A.
PY - 2020
Y1 - 2020
N2 - CLE peptides are important signaling molecules that regulate the balance of proliferation and differentiation of plant cells. In this work, we conjugated gold nanospheres (15 nm) with a chemically synthesized peptide (12 amino acid residues) that the amino acid sequence corresponds to the natural peptides CLE41/44. The "golden number" for CLE41/44 was 2 Μg/mL. A stable conjugate was used to produce rabbit polyclonal antibody. By the method of dot analysis, the presence of antibodies to CLE41/44 was detected in antiserum. Using Western blot and ELISA methods, the reaction of the obtained antibody with a conjugate of CLE41/44 with BSA molecules was demonstrated. The obtained antibody is can potentially be used to the quantitative determination and identification of the localization of peptides in plant tissues.
AB - CLE peptides are important signaling molecules that regulate the balance of proliferation and differentiation of plant cells. In this work, we conjugated gold nanospheres (15 nm) with a chemically synthesized peptide (12 amino acid residues) that the amino acid sequence corresponds to the natural peptides CLE41/44. The "golden number" for CLE41/44 was 2 Μg/mL. A stable conjugate was used to produce rabbit polyclonal antibody. By the method of dot analysis, the presence of antibodies to CLE41/44 was detected in antiserum. Using Western blot and ELISA methods, the reaction of the obtained antibody with a conjugate of CLE41/44 with BSA molecules was demonstrated. The obtained antibody is can potentially be used to the quantitative determination and identification of the localization of peptides in plant tissues.
M3 - Abstract
SP - 1145716
T2 - <br/>7TH INTERNATIONAL SYMPOSIUM ON OPTICS AND BIOPHOTONICS: OPTICAL AND NANO-TECHNOLOGIES FOR BIOLOGY AND MEDICINE
Y2 - 23 September 2019 through 27 September 2019
ER -
ID: 78533248