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Nucleosomal structure and histone H1 subfractional composition of pea (Pisum sativum) root nodules, radicles and callus chromatin. / Bers, E. P.; Singh, N. P.; Pardonen, V. A.; Lutova, L. A.; Zalensky, A. O.

In: Plant Molecular Biology, Vol. 20, No. 6, 12.1992, p. 1089-1096.

Research output: Contribution to journal › Article › peer-review

Harvard

Bers, EP, Singh, NP, Pardonen, VA, Lutova, LA & Zalensky, AO 1992, 'Nucleosomal structure and histone H1 subfractional composition of pea (Pisum sativum) root nodules, radicles and callus chromatin', Plant Molecular Biology, vol. 20, no. 6, pp. 1089-1096. https://doi.org/10.1007/BF00028895

APA

Bers, E. P., Singh, N. P., Pardonen, V. A., Lutova, L. A., & Zalensky, A. O. (1992). Nucleosomal structure and histone H1 subfractional composition of pea (Pisum sativum) root nodules, radicles and callus chromatin. Plant Molecular Biology, 20(6), 1089-1096. https://doi.org/10.1007/BF00028895

Vancouver

Bers EP, Singh NP, Pardonen VA, Lutova LA, Zalensky AO. Nucleosomal structure and histone H1 subfractional composition of pea (Pisum sativum) root nodules, radicles and callus chromatin. Plant Molecular Biology. 1992 Dec;20(6):1089-1096. https://doi.org/10.1007/BF00028895

Author

Bers, E. P. ; Singh, N. P. ; Pardonen, V. A. ; Lutova, L. A. ; Zalensky, A. O. / Nucleosomal structure and histone H1 subfractional composition of pea (Pisum sativum) root nodules, radicles and callus chromatin. In: Plant Molecular Biology. 1992 ; Vol. 20, No. 6. pp. 1089-1096.

BibTeX

@article{d4ef91e32dcd421ba7b498f947dfc010,
title = "Nucleosomal structure and histone H1 subfractional composition of pea (Pisum sativum) root nodules, radicles and callus chromatin",
abstract = "Higher-order packaging of DNA in chromatin structures could be an essential step in the complex chain of events leading to activation/repression of eukaryotic gene expression. With the goal to investigate this aspect of transcriptional regulation of plant genes involved in symbiotic interactions between legumes and rhizobia we analyze here the molecular parameters of chromatin structure in functioning root nodules, callus and radicles of pea. Morphological intactness and the typical nucleosomal organization are preserved in purified nuclei isolated from all three sources. The calculated values of nucleosomal repeat changed from 185±5 bp in the nuclei of radicles to 168±5 bp and 195±6 bp in nodules and callus respectively. The observed changes are due to alterations in linker DNA lengths. The core histones are identical in all cases, but the subfractional composition of H1 linker histone is subjected to quantitative alterations. The most pronounced is the several-fold increase in content of the lowest-molecular-weight subfraction H1-6 which takes place during nodule development.",
keywords = "chromatin, histone H1, nodule development, nucleosomal repeat, plant nuclei",
author = "Bers, {E. P.} and Singh, {N. P.} and Pardonen, {V. A.} and Lutova, {L. A.} and Zalensky, {A. O.}",
year = "1992",
month = dec,
doi = "10.1007/BF00028895",
language = "English",
volume = "20",
pages = "1089--1096",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Nature",
number = "6",

}

RIS

TY - JOUR

T1 - Nucleosomal structure and histone H1 subfractional composition of pea (Pisum sativum) root nodules, radicles and callus chromatin

AU - Bers, E. P.

AU - Singh, N. P.

AU - Pardonen, V. A.

AU - Lutova, L. A.

AU - Zalensky, A. O.

PY - 1992/12

Y1 - 1992/12

N2 - Higher-order packaging of DNA in chromatin structures could be an essential step in the complex chain of events leading to activation/repression of eukaryotic gene expression. With the goal to investigate this aspect of transcriptional regulation of plant genes involved in symbiotic interactions between legumes and rhizobia we analyze here the molecular parameters of chromatin structure in functioning root nodules, callus and radicles of pea. Morphological intactness and the typical nucleosomal organization are preserved in purified nuclei isolated from all three sources. The calculated values of nucleosomal repeat changed from 185±5 bp in the nuclei of radicles to 168±5 bp and 195±6 bp in nodules and callus respectively. The observed changes are due to alterations in linker DNA lengths. The core histones are identical in all cases, but the subfractional composition of H1 linker histone is subjected to quantitative alterations. The most pronounced is the several-fold increase in content of the lowest-molecular-weight subfraction H1-6 which takes place during nodule development.

AB - Higher-order packaging of DNA in chromatin structures could be an essential step in the complex chain of events leading to activation/repression of eukaryotic gene expression. With the goal to investigate this aspect of transcriptional regulation of plant genes involved in symbiotic interactions between legumes and rhizobia we analyze here the molecular parameters of chromatin structure in functioning root nodules, callus and radicles of pea. Morphological intactness and the typical nucleosomal organization are preserved in purified nuclei isolated from all three sources. The calculated values of nucleosomal repeat changed from 185±5 bp in the nuclei of radicles to 168±5 bp and 195±6 bp in nodules and callus respectively. The observed changes are due to alterations in linker DNA lengths. The core histones are identical in all cases, but the subfractional composition of H1 linker histone is subjected to quantitative alterations. The most pronounced is the several-fold increase in content of the lowest-molecular-weight subfraction H1-6 which takes place during nodule development.

KW - chromatin

KW - histone H1

KW - nodule development

KW - nucleosomal repeat

KW - plant nuclei

UR - http://www.scopus.com/inward/record.url?scp=0027022421&partnerID=8YFLogxK

U2 - 10.1007/BF00028895

DO - 10.1007/BF00028895

M3 - Article

C2 - 1463843

AN - SCOPUS:0027022421

VL - 20

SP - 1089

EP - 1096

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 6

ER -

ID: 95236544