Research output: Contribution to journal › Article
Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II. / Pochukalina, G. N.; Ilicheva, N. V; Podgornaya, O. I.; Voronin, A. P.
In: Molecular Cytogenetics, Vol. 9, No. 50, 2016, p. 1-14.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II
AU - Pochukalina, G. N.
AU - Ilicheva, N. V
AU - Podgornaya, O. I.
AU - Voronin, A. P.
PY - 2016
Y1 - 2016
N2 - Background: During the final stages of oocyte development, all chromosomes join in a limited nuclear volume for the final formation of a single complex chromatin structure – the karyosphere. In the majority of mammalian species, the chromosomes surround a round protein/fibrillar body known as the central body, or nucleolus-like body (NLB). Nothing seems to unite the inner portion of the karyosphere with the nucleolus except position at its remnants. Nevertheless, in this study we will use term NLB as the conventional one for karyosphere with the central body. At the morphological level, NLBs consist of tightly-packed fibres of 6–10 nm. The biochemical structure of this dense, compact NLB fibre centre remains uncertain. Results: The aim of this study was to determine which proteins represent the NLB components at final stages of karyosphere formation in mouse oogenesis. To determine this, three antibodies (ABs) have been examined against different actin epitopes. Examination of both ABs against the actin N-end
AB - Background: During the final stages of oocyte development, all chromosomes join in a limited nuclear volume for the final formation of a single complex chromatin structure – the karyosphere. In the majority of mammalian species, the chromosomes surround a round protein/fibrillar body known as the central body, or nucleolus-like body (NLB). Nothing seems to unite the inner portion of the karyosphere with the nucleolus except position at its remnants. Nevertheless, in this study we will use term NLB as the conventional one for karyosphere with the central body. At the morphological level, NLBs consist of tightly-packed fibres of 6–10 nm. The biochemical structure of this dense, compact NLB fibre centre remains uncertain. Results: The aim of this study was to determine which proteins represent the NLB components at final stages of karyosphere formation in mouse oogenesis. To determine this, three antibodies (ABs) have been examined against different actin epitopes. Examination of both ABs against the actin N-end
KW - Mouse oogenesis Morphology Karyosphere Nucleolus-like body Immunofluorescence
U2 - 10.1186/s13039-016-0259-3
DO - 10.1186/s13039-016-0259-3
M3 - Article
VL - 9
SP - 1
EP - 14
JO - Molecular Cytogenetics
JF - Molecular Cytogenetics
SN - 1755-8166
IS - 50
ER -
ID: 7630714