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Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II. / Pochukalina, G. N.; Ilicheva, N. V; Podgornaya, O. I.; Voronin, A. P.

In: Molecular Cytogenetics, Vol. 9, No. 50, 2016, p. 1-14.

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Pochukalina, G. N. ; Ilicheva, N. V ; Podgornaya, O. I. ; Voronin, A. P. / Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II. In: Molecular Cytogenetics. 2016 ; Vol. 9, No. 50. pp. 1-14.

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@article{758281ec208d485cac90b83c28aff909,
title = "Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II",
abstract = "Background: During the final stages of oocyte development, all chromosomes join in a limited nuclear volume for the final formation of a single complex chromatin structure – the karyosphere. In the majority of mammalian species, the chromosomes surround a round protein/fibrillar body known as the central body, or nucleolus-like body (NLB). Nothing seems to unite the inner portion of the karyosphere with the nucleolus except position at its remnants. Nevertheless, in this study we will use term NLB as the conventional one for karyosphere with the central body. At the morphological level, NLBs consist of tightly-packed fibres of 6–10 nm. The biochemical structure of this dense, compact NLB fibre centre remains uncertain. Results: The aim of this study was to determine which proteins represent the NLB components at final stages of karyosphere formation in mouse oogenesis. To determine this, three antibodies (ABs) have been examined against different actin epitopes. Examination of both ABs against the actin N-end",
keywords = "Mouse oogenesis Morphology Karyosphere Nucleolus-like body Immunofluorescence",
author = "Pochukalina, {G. N.} and Ilicheva, {N. V} and Podgornaya, {O. I.} and Voronin, {A. P.}",
year = "2016",
doi = "10.1186/s13039-016-0259-3",
language = "English",
volume = "9",
pages = "1--14",
journal = "Molecular Cytogenetics",
issn = "1755-8166",
publisher = "BioMed Central Ltd.",
number = "50",

}

RIS

TY - JOUR

T1 - Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II

AU - Pochukalina, G. N.

AU - Ilicheva, N. V

AU - Podgornaya, O. I.

AU - Voronin, A. P.

PY - 2016

Y1 - 2016

N2 - Background: During the final stages of oocyte development, all chromosomes join in a limited nuclear volume for the final formation of a single complex chromatin structure – the karyosphere. In the majority of mammalian species, the chromosomes surround a round protein/fibrillar body known as the central body, or nucleolus-like body (NLB). Nothing seems to unite the inner portion of the karyosphere with the nucleolus except position at its remnants. Nevertheless, in this study we will use term NLB as the conventional one for karyosphere with the central body. At the morphological level, NLBs consist of tightly-packed fibres of 6–10 nm. The biochemical structure of this dense, compact NLB fibre centre remains uncertain. Results: The aim of this study was to determine which proteins represent the NLB components at final stages of karyosphere formation in mouse oogenesis. To determine this, three antibodies (ABs) have been examined against different actin epitopes. Examination of both ABs against the actin N-end

AB - Background: During the final stages of oocyte development, all chromosomes join in a limited nuclear volume for the final formation of a single complex chromatin structure – the karyosphere. In the majority of mammalian species, the chromosomes surround a round protein/fibrillar body known as the central body, or nucleolus-like body (NLB). Nothing seems to unite the inner portion of the karyosphere with the nucleolus except position at its remnants. Nevertheless, in this study we will use term NLB as the conventional one for karyosphere with the central body. At the morphological level, NLBs consist of tightly-packed fibres of 6–10 nm. The biochemical structure of this dense, compact NLB fibre centre remains uncertain. Results: The aim of this study was to determine which proteins represent the NLB components at final stages of karyosphere formation in mouse oogenesis. To determine this, three antibodies (ABs) have been examined against different actin epitopes. Examination of both ABs against the actin N-end

KW - Mouse oogenesis Morphology Karyosphere Nucleolus-like body Immunofluorescence

U2 - 10.1186/s13039-016-0259-3

DO - 10.1186/s13039-016-0259-3

M3 - Article

VL - 9

SP - 1

EP - 14

JO - Molecular Cytogenetics

JF - Molecular Cytogenetics

SN - 1755-8166

IS - 50

ER -

ID: 7630714