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Nuclear actin depolymerisation in transcriptionally active avian and amphibian oocytes leads to collapse of intranuclear structures. / Maslova, A.; Krasikova, A.

In: Nucleus, Vol. 3, No. 3, 2012, p. 300-311.

Research output: Contribution to journal › Article

Harvard

Maslova, A & Krasikova, A 2012, 'Nuclear actin depolymerisation in transcriptionally active avian and amphibian oocytes leads to collapse of intranuclear structures', Nucleus, vol. 3, no. 3, pp. 300-311. https://doi.org/10.4161/nucl.20393

APA

Maslova, A., & Krasikova, A. (2012). Nuclear actin depolymerisation in transcriptionally active avian and amphibian oocytes leads to collapse of intranuclear structures. Nucleus, 3(3), 300-311. https://doi.org/10.4161/nucl.20393

Vancouver

Maslova A, Krasikova A. Nuclear actin depolymerisation in transcriptionally active avian and amphibian oocytes leads to collapse of intranuclear structures. Nucleus. 2012;3(3):300-311. https://doi.org/10.4161/nucl.20393

Author

Maslova, A. ; Krasikova, A. / Nuclear actin depolymerisation in transcriptionally active avian and amphibian oocytes leads to collapse of intranuclear structures. In: Nucleus. 2012 ; Vol. 3, No. 3. pp. 300-311.

BibTeX

@article{47682b8d6714472980ab5c83b87dea2d,
title = "Nuclear actin depolymerisation in transcriptionally active avian and amphibian oocytes leads to collapse of intranuclear structures",
abstract = "Actin, which is normally depleted in the nuclei of somatic cells, accumulates in high amounts in giant nuclei of amphibian oocytes. The supramolecular organization and functions of this nuclear pool of actin in growing vertebrate oocyte are controversial. Here, we investigated the role of nuclear actin in the maintenance of the spatial architecture of intranuclear structures in avian and amphibian growing oocytes. A meshwork of filamentous actin was not detected in freshly isolated or fixed oocyte nuclei of Xenopus, chicken, or quail. We found that the actin meshwork inside the oocyte nucleus could be induced by phalloidin treatment. Actin polymerisation is demonstrated to be required to stabilize the specific spatial organization of nuclear structures in avian and amphibian growing oocytes. In experiments with the actin depolymerising drugs cytochalasin D and latrunculin A, we showed that disassembly of nuclear actin polymers led to chromosome condensation and their transportation to a limited space within t",
keywords = "cytochalasin, cell nucleus, genome architecture, germinal vesicle, karyosphere, lampbrush chromosomes, latrunculin, nuclear actin, nuclear bodies, oogenesis, transcription",
author = "A. Maslova and A. Krasikova",
year = "2012",
doi = "10.4161/nucl.20393",
language = "English",
volume = "3",
pages = "300--311",
journal = "Nucleus",
issn = "1949-1034",
publisher = "Landes Bioscience",
number = "3",

}

RIS

TY - JOUR

T1 - Nuclear actin depolymerisation in transcriptionally active avian and amphibian oocytes leads to collapse of intranuclear structures

AU - Maslova, A.

AU - Krasikova, A.

PY - 2012

Y1 - 2012

N2 - Actin, which is normally depleted in the nuclei of somatic cells, accumulates in high amounts in giant nuclei of amphibian oocytes. The supramolecular organization and functions of this nuclear pool of actin in growing vertebrate oocyte are controversial. Here, we investigated the role of nuclear actin in the maintenance of the spatial architecture of intranuclear structures in avian and amphibian growing oocytes. A meshwork of filamentous actin was not detected in freshly isolated or fixed oocyte nuclei of Xenopus, chicken, or quail. We found that the actin meshwork inside the oocyte nucleus could be induced by phalloidin treatment. Actin polymerisation is demonstrated to be required to stabilize the specific spatial organization of nuclear structures in avian and amphibian growing oocytes. In experiments with the actin depolymerising drugs cytochalasin D and latrunculin A, we showed that disassembly of nuclear actin polymers led to chromosome condensation and their transportation to a limited space within t

AB - Actin, which is normally depleted in the nuclei of somatic cells, accumulates in high amounts in giant nuclei of amphibian oocytes. The supramolecular organization and functions of this nuclear pool of actin in growing vertebrate oocyte are controversial. Here, we investigated the role of nuclear actin in the maintenance of the spatial architecture of intranuclear structures in avian and amphibian growing oocytes. A meshwork of filamentous actin was not detected in freshly isolated or fixed oocyte nuclei of Xenopus, chicken, or quail. We found that the actin meshwork inside the oocyte nucleus could be induced by phalloidin treatment. Actin polymerisation is demonstrated to be required to stabilize the specific spatial organization of nuclear structures in avian and amphibian growing oocytes. In experiments with the actin depolymerising drugs cytochalasin D and latrunculin A, we showed that disassembly of nuclear actin polymers led to chromosome condensation and their transportation to a limited space within t

KW - cytochalasin

KW - cell nucleus

KW - genome architecture

KW - germinal vesicle

KW - karyosphere

KW - lampbrush chromosomes

KW - latrunculin

KW - nuclear actin

KW - nuclear bodies

KW - oogenesis

KW - transcription

U2 - 10.4161/nucl.20393

DO - 10.4161/nucl.20393

M3 - Article

VL - 3

SP - 300

EP - 311

JO - Nucleus

JF - Nucleus

SN - 1949-1034

IS - 3

ER -

ID: 5324897