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Nonsense mutations in the yeast SUP35 gene affect the [PSI+] prion propagation. / Trubitsina, Nina P.; Zemlyanko, Olga M.; Bondarev, Stanislav A.; Zhouravleva, Galina A.
In: International Journal of Molecular Sciences, Vol. 21, No. 5, 1648, 03.2020.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Nonsense mutations in the yeast SUP35 gene affect the [PSI+] prion propagation
AU - Trubitsina, Nina P.
AU - Zemlyanko, Olga M.
AU - Bondarev, Stanislav A.
AU - Zhouravleva, Galina A.
N1 - Funding Information: Funding: This work was supported by grants from Russian Science Foundation [grant 18‐14‐00050] (plasmid construction, fluorescence microscopy, mutation analysis in haploids) and Russian Foundation for Basic Research [grant 17‐54‐150002] (individual mutation analysis in diploids). Technical help was provided by Resource Center “Development of Molecular and Cell Technologies” of St. Petersburg State University. Authors declare no conflict of interest. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/3
Y1 - 2020/3
N2 - The essential SUP35 gene encodes yeast translation termination factor eRF3. Previously, we isolated nonsense mutations sup35-n and proposed that the viability of such mutants can be explained by readthrough of the premature stop codon. Such mutations, as well as the prion [PSI+], can appear in natural yeast populations, and their combinations may have different effects on the cells. Here, we analyze the effects of the compatibility of sup35-n mutations with the [PSI+] prion in haploid and diploid cells. We demonstrated that sup35-n mutations are incompatible with the [PSI+] prion, leading to lethality of sup35-n [PSI+] haploid cells. In diploid cells the compatibility of [PSI+] with sup35-n depends on how the corresponding diploid was obtained. Nonsense mutations sup35-21, sup35-74, and sup35-218 are compatible with the [PSI+] prion in diploid strains, but affect [PSI+] properties and lead to the formation of new prion variant. The only mutation that could replace the SUP35 wild-type allele in both haploid and diploid [PSI+] strains, sup35-240, led to the prion loss. Possibly, short Sup351–55 protein, produced from the sup35-240 allele, is included in Sup35 aggregates and destabilize them. Alternatively, single molecules of Sup351–55 can stick to aggregate ends, and thus interrupt the fibril growth. Thus, we can conclude that sup35-240 mutation prevents [PSI+] propagation and can be considered as a new pnm mutation.
AB - The essential SUP35 gene encodes yeast translation termination factor eRF3. Previously, we isolated nonsense mutations sup35-n and proposed that the viability of such mutants can be explained by readthrough of the premature stop codon. Such mutations, as well as the prion [PSI+], can appear in natural yeast populations, and their combinations may have different effects on the cells. Here, we analyze the effects of the compatibility of sup35-n mutations with the [PSI+] prion in haploid and diploid cells. We demonstrated that sup35-n mutations are incompatible with the [PSI+] prion, leading to lethality of sup35-n [PSI+] haploid cells. In diploid cells the compatibility of [PSI+] with sup35-n depends on how the corresponding diploid was obtained. Nonsense mutations sup35-21, sup35-74, and sup35-218 are compatible with the [PSI+] prion in diploid strains, but affect [PSI+] properties and lead to the formation of new prion variant. The only mutation that could replace the SUP35 wild-type allele in both haploid and diploid [PSI+] strains, sup35-240, led to the prion loss. Possibly, short Sup351–55 protein, produced from the sup35-240 allele, is included in Sup35 aggregates and destabilize them. Alternatively, single molecules of Sup351–55 can stick to aggregate ends, and thus interrupt the fibril growth. Thus, we can conclude that sup35-240 mutation prevents [PSI+] propagation and can be considered as a new pnm mutation.
KW - Nonsense mutations
KW - Prion
KW - S.cerevisiae
KW - Sup35
KW - Translation termination
KW - Yeast
KW - [PSI]
KW - DETERMINANT
KW - [PSI+]
KW - translation termination
KW - SACCHAROMYCES-CEREVISIAE
KW - yeast
KW - OVEREXPRESSION
KW - S
KW - PROTEIN EXTRACTION
KW - SUP45
KW - NON-MENDELIAN FACTOR
KW - PSI(+)
KW - cerevisiae
KW - prion
KW - nonsense mutations
KW - DEPENDENT LETHALITY
KW - EXPRESSION
KW - TRANSLATION TERMINATION
UR - http://www.scopus.com/inward/record.url?scp=85080881356&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/f2c454ef-9cf8-341a-a1c1-11630233c587/
U2 - 10.3390/ijms21051648
DO - 10.3390/ijms21051648
M3 - Article
C2 - 32121268
AN - SCOPUS:85080881356
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 5
M1 - 1648
ER -
ID: 70378249