TY - JOUR

T1 - Non-enzymatic voltammetric sensor for histamine determination in saliva for Helicobacter Pylori screening

AU - Solokhin, Alexandr Yu.

AU - Kolomina, Elena O.

AU - Haurylenka, Tatsiana

AU - Navolotskaya, Daria

AU - Selyutin, Artem A.

AU - Ermakov, Sergey S.

N1 - Solokhin, Alexandr Yu. and Kolomina, Elena O. and Haurylenka, Tatsiana and Navolotskaya, Daria and Selyutin, Artem A. and Ermakov, Sergey S., Non-Enzymatic Voltammetric Sensor for Histamine Determination in Saliva for Helicobacter Pylori Screening. Available at SSRN: https://ssrn.com/abstract=4143131 or http://dx.doi.org/10.2139/ssrn.4143131

PY - 2022

Y1 - 2022

N2 - Еvaluation of histamine levels in saliva can be used in characterization of Helicobacter pylori extragastric manifestations. Therefore, a sensitive, simple, and low-cost method for determining histamine concentrations is needed for point-of-care diagnostics. In this work, we studied the use of glassy carbon disk electrode, bulk graphite composite electrode and graphite screen-printed electrode modified with CuO nanoparticles and CuSO4 towards histamine quantification by cyclic voltammetry. The best results were obtained using the screen-printed sensor, whose working electrode had been modified by CuSO4 aqueous solution and covered with Nafion-like polymer film. The developed sensor showed a 5·10-9 M detection limit with a broad linear range from 5·10-9 M till 1·10-6 M of histamine in PBS, which is enough for reliable saliva analysis after the simple sample preparation. The analysis of real saliva samples revealed no significant interfering matrix effect. The time of analysis, including sample preparation, doesn’t exceed 15 minutes.

AB - Еvaluation of histamine levels in saliva can be used in characterization of Helicobacter pylori extragastric manifestations. Therefore, a sensitive, simple, and low-cost method for determining histamine concentrations is needed for point-of-care diagnostics. In this work, we studied the use of glassy carbon disk electrode, bulk graphite composite electrode and graphite screen-printed electrode modified with CuO nanoparticles and CuSO4 towards histamine quantification by cyclic voltammetry. The best results were obtained using the screen-printed sensor, whose working electrode had been modified by CuSO4 aqueous solution and covered with Nafion-like polymer film. The developed sensor showed a 5·10-9 M detection limit with a broad linear range from 5·10-9 M till 1·10-6 M of histamine in PBS, which is enough for reliable saliva analysis after the simple sample preparation. The analysis of real saliva samples revealed no significant interfering matrix effect. The time of analysis, including sample preparation, doesn’t exceed 15 minutes.

KW - Histamine

KW - voltammetric sensor

KW - point-of-care diagnostics

KW - saliva

KW - periodontitis

UR - https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4143131

UR - https://www.mendeley.com/catalogue/653b7874-6965-321f-ad53-ef3ad518aa98/

U2 - 10.2139/ssrn.4143131

DO - 10.2139/ssrn.4143131

M3 - Article

JO - Talanta

JF - Talanta

SN - 0039-9140

ER -