TY - JOUR

T1 - Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato

AU - Ганчева, Мария Семеновна

AU - Лутова, Людмила Алексеевна

PY - 2023/10/3

Y1 - 2023/10/3

N2 - In potato, high levels of nitrogen (N) can lead to excessive vegetative growth at the expense of tuber development, resulting in lower yield and poor-quality tubers. We found that Solanum tuberosum CLE4 ( StCLE4) is expressed most strongly in the roots grown in N-rich media, and it positively regulates potato root growth under N-deficient conditions. We noted that StCLE4 functions as a negative regulator of normal shoot apex development similar to CLV3 in Arabidopsis. Transcriptomic analysis revealed that overexpression of StCLE4 resulted in the repression of the StIT1 gene, a regulator of potato tuber initiation. StCLE4-overexpressing stolons were converted into branches, that were similar to a mild phenotype of the it1 ( identity of tuber 1) mutant. We also found that NIN-like proteins, key regulators of nitrate signaling bind to the regulatory sequence of StIT1 in a yeast one-hybrid assay. Taken together, our findings suggest that StCLE4 regulates shoot, root, and stolon growth in potato.

AB - In potato, high levels of nitrogen (N) can lead to excessive vegetative growth at the expense of tuber development, resulting in lower yield and poor-quality tubers. We found that Solanum tuberosum CLE4 ( StCLE4) is expressed most strongly in the roots grown in N-rich media, and it positively regulates potato root growth under N-deficient conditions. We noted that StCLE4 functions as a negative regulator of normal shoot apex development similar to CLV3 in Arabidopsis. Transcriptomic analysis revealed that overexpression of StCLE4 resulted in the repression of the StIT1 gene, a regulator of potato tuber initiation. StCLE4-overexpressing stolons were converted into branches, that were similar to a mild phenotype of the it1 ( identity of tuber 1) mutant. We also found that NIN-like proteins, key regulators of nitrate signaling bind to the regulatory sequence of StIT1 in a yeast one-hybrid assay. Taken together, our findings suggest that StCLE4 regulates shoot, root, and stolon growth in potato.

KW - CLE peptide

KW - CLE4

KW - Identity of Tuber1 (IT1)

KW - NIN-like proteins (NLPs)

KW - nitrate

KW - nitrate-responsive elements (NREs)

KW - potato

KW - shoot apical meristem (SAM)

KW - tuber

UR - https://www.mendeley.com/catalogue/19430846-cb33-31e9-9fe2-2084510a2122/

U2 - 10.3390/plants12193468

DO - 10.3390/plants12193468

M3 - Article

C2 - 37836208

VL - 12

JO - Plants

JF - Plants

SN - 2223-7747

IS - 19

M1 - 3468

ER -