Research output: Contribution to journal › Article › peer-review
Nitrate-induced cle peptide systemically inhibits nodulation in medicago truncatula. / Lebedeva, Maria; Azarakhsh, Mahboobeh; Yashenkova, Yaroslavna; Lutova, Lyudmila.
In: Plants, Vol. 9, No. 11, 1456, 11.2020, p. 1-12.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Nitrate-induced cle peptide systemically inhibits nodulation in medicago truncatula
AU - Lebedeva, Maria
AU - Azarakhsh, Mahboobeh
AU - Yashenkova, Yaroslavna
AU - Lutova, Lyudmila
N1 - Funding Information: Funding: This work was supported by Russian Scientific Foundation project no. 16-16-10011, Russian Foundation for Basic Research (RFBR) projects 20-016-00129, 19-016-00177, and grant from Saint-Petersburg State University ID 60256785. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11
Y1 - 2020/11
N2 - Legume plants form nitrogen-fixing nodules in symbiosis with soil bacteria rhizobia. The number of symbiotic nodules is controlled at the whole-plant level with autoregulation of nodulation (AON), which includes a shoot-acting CLV1-like receptor kinase and mobile CLE (CLAVATA3/ENDOSPERM SURROUNDING REGION-related) peptides that are produced in the root in response to rhizobia inoculation. In addition to rhizobia-induced CLE peptides, nitrate-induced CLE genes have been identified in Lotus japonicus and Glycine max, which inhibited nodulation when overexpressed. However, nitrate-induced CLE genes that systemically suppress nodulation in AON-dependent manner have not been identified in Medicago truncatula. Here, we found that MtCLE35 expression is activated by both rhizobia inoculation and nitrate treatment in M. truncatula, similarly to L. japonicus CLE genes. Moreover, we found that MtCLE35 systemically suppresses nodulation in AON-dependent manner, suggesting that MtCLE35 may mediate nitrate-induced inhibition of nodulation in M. truncatula.
AB - Legume plants form nitrogen-fixing nodules in symbiosis with soil bacteria rhizobia. The number of symbiotic nodules is controlled at the whole-plant level with autoregulation of nodulation (AON), which includes a shoot-acting CLV1-like receptor kinase and mobile CLE (CLAVATA3/ENDOSPERM SURROUNDING REGION-related) peptides that are produced in the root in response to rhizobia inoculation. In addition to rhizobia-induced CLE peptides, nitrate-induced CLE genes have been identified in Lotus japonicus and Glycine max, which inhibited nodulation when overexpressed. However, nitrate-induced CLE genes that systemically suppress nodulation in AON-dependent manner have not been identified in Medicago truncatula. Here, we found that MtCLE35 expression is activated by both rhizobia inoculation and nitrate treatment in M. truncatula, similarly to L. japonicus CLE genes. Moreover, we found that MtCLE35 systemically suppresses nodulation in AON-dependent manner, suggesting that MtCLE35 may mediate nitrate-induced inhibition of nodulation in M. truncatula.
KW - Autoregulation of nodulation
KW - CLE
KW - CLV1-like kinase
KW - Nitrate
KW - Symbiotic nodules
UR - http://www.scopus.com/inward/record.url?scp=85094606636&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/9cf63bba-368f-327f-999b-c6b39fac2fe3/
U2 - 10.3390/plants9111456
DO - 10.3390/plants9111456
M3 - Article
AN - SCOPUS:85094606636
VL - 9
SP - 1
EP - 12
JO - Plants
JF - Plants
SN - 2223-7747
IS - 11
M1 - 1456
ER -
ID: 71156423