Nicotinamide riboside has protective effects in a rat model of mesenteric ischaemia-reperfusion. / Toropova, Yana G.; Pechnikova, Nadezdha A.; Zelinskaya, Irina A.; Zhuravsky, Sergey G.; Kornyushin, Oleg V.; Gonchar, Alina I.; Ivkin, Dmitry Y.; Leonova, Yulia V.; Karev, Vadim E.; Karabak, Irina A.
In: International Journal of Experimental Pathology, Vol. 99, No. 6, 12.2018, p. 304-311.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Nicotinamide riboside has protective effects in a rat model of mesenteric ischaemia-reperfusion
AU - Toropova, Yana G.
AU - Pechnikova, Nadezdha A.
AU - Zelinskaya, Irina A.
AU - Zhuravsky, Sergey G.
AU - Kornyushin, Oleg V.
AU - Gonchar, Alina I.
AU - Ivkin, Dmitry Y.
AU - Leonova, Yulia V.
AU - Karev, Vadim E.
AU - Karabak, Irina A.
N1 - Publisher Copyright: © 2019 The Authors. International Journal of Experimental Pathology © 2019 International Journal of Experimental Pathology
PY - 2018/12
Y1 - 2018/12
N2 - Acute mesenteric ischaemia is a syndrome caused by inadequate blood flow through the mesenteric vessels, resulting in ischaemia and eventual gangrene of the bowel wall. Although relatively rare, it is a potentially life-threatening condition. The maintenance of haemodynamic stability, along with adequate oxygen saturation, and the correction of any electrolyte imbalance, are of the utmost importance. However, nicotinamide adenine dinucleotide (NAD) biosynthesis modulation by precursor introduction can also be a powerful tool for preventing injury. Nicotinamide riboside is a pyridine-nucleoside form of vitamin B3 that functions as a precursor to NAD + . The present study investigated nicotinamide riboside's effect on endothelium functional state, microcirculation and intestinal morphology in acute mesenteric ischaemia and reperfusion. Mesenteric ischaemia was simulated after the adaptation period (15 minutes) by occluding the superior mesenteric artery for 60 minutes, followed by a reperfusion period of 30 minutes. The functional state of intestinal microcirculation was evaluated by laser Doppler flowmetry. Endothelial functional activity was studied by using wire myography. Intestinal samples were stained with haematoxylin and eosin for histological analysis. The results revealed that nicotinamide riboside protects the intestinal wall from ischaemia-reperfusion injury, as well as improving the relaxation function of mesenteric vessels. Nicotinamide riboside's protective effect in small intestine ischaemia-reperfusion can be used to reduce ischaemia-reperfusion injury, as well as to preserve intestinal grafts until transplant.
AB - Acute mesenteric ischaemia is a syndrome caused by inadequate blood flow through the mesenteric vessels, resulting in ischaemia and eventual gangrene of the bowel wall. Although relatively rare, it is a potentially life-threatening condition. The maintenance of haemodynamic stability, along with adequate oxygen saturation, and the correction of any electrolyte imbalance, are of the utmost importance. However, nicotinamide adenine dinucleotide (NAD) biosynthesis modulation by precursor introduction can also be a powerful tool for preventing injury. Nicotinamide riboside is a pyridine-nucleoside form of vitamin B3 that functions as a precursor to NAD + . The present study investigated nicotinamide riboside's effect on endothelium functional state, microcirculation and intestinal morphology in acute mesenteric ischaemia and reperfusion. Mesenteric ischaemia was simulated after the adaptation period (15 minutes) by occluding the superior mesenteric artery for 60 minutes, followed by a reperfusion period of 30 minutes. The functional state of intestinal microcirculation was evaluated by laser Doppler flowmetry. Endothelial functional activity was studied by using wire myography. Intestinal samples were stained with haematoxylin and eosin for histological analysis. The results revealed that nicotinamide riboside protects the intestinal wall from ischaemia-reperfusion injury, as well as improving the relaxation function of mesenteric vessels. Nicotinamide riboside's protective effect in small intestine ischaemia-reperfusion can be used to reduce ischaemia-reperfusion injury, as well as to preserve intestinal grafts until transplant.
KW - acute mesenteric ischaemia
KW - ischaemia-reperfusion injury
KW - microcirculation
KW - nicotinamide riboside
KW - wire myography
UR - http://www.scopus.com/inward/record.url?scp=85061918883&partnerID=8YFLogxK
U2 - 10.1111/iep.12302
DO - 10.1111/iep.12302
M3 - Article
C2 - 30793416
AN - SCOPUS:85061918883
VL - 99
SP - 304
EP - 311
JO - International Journal of Experimental Pathology
JF - International Journal of Experimental Pathology
SN - 0959-9673
IS - 6
ER -
ID: 85244950