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Nicotiana glauca whole-genome investigation for cT-DNA study. / Хафизова, Галина Васильевна; Добрынин, Павел Владимирович; Полев, Дмитрий Евгеньевич; Матвеева, Татьяна Валерьевна.

In: BMC Research Notes, Vol. 11, No. 1, 18, 12.01.2018.

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@article{2c353cec9469495f93c3aadcae265f35,
title = "Nicotiana glauca whole-genome investigation for cT-DNA study",
abstract = "Objective: Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. Data description: We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion. No evidence was acquired to support idea of multiple T-DNA insertions in the N. glauca genome. Our data is the first comprehensive de novo assembly of tree tobacco and provide valuable information for researches in pharmacological and in phylogenetic fields.",
keywords = "Cellular T-DNA, Genome assembly, Nicotiana glauca, Whole genome sequencing, High-Throughput Nucleotide Sequencing/methods, Tobacco/genetics, Gene Transfer, Horizontal/genetics, DNA, Bacterial/genetics, Genome, Plant/genetics, Sequence Analysis, DNA/methods",
author = "Хафизова, {Галина Васильевна} and Добрынин, {Павел Владимирович} and Полев, {Дмитрий Евгеньевич} and Матвеева, {Татьяна Валерьевна}",
note = "Funding Information: This paper was supported by a Grant to Tatiana Matveeva from the Russian Science Foundation 16-16-10010 and a Grant from Saint Petersburg State University №1.52.1647.2016.",
year = "2018",
month = jan,
day = "12",
doi = "10.1101/211482",
language = "English",
volume = "11",
journal = "BMC Research Notes",
issn = "1756-0500",
publisher = "BioMed Central Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Nicotiana glauca whole-genome investigation for cT-DNA study

AU - Хафизова, Галина Васильевна

AU - Добрынин, Павел Владимирович

AU - Полев, Дмитрий Евгеньевич

AU - Матвеева, Татьяна Валерьевна

N1 - Funding Information: This paper was supported by a Grant to Tatiana Matveeva from the Russian Science Foundation 16-16-10010 and a Grant from Saint Petersburg State University №1.52.1647.2016.

PY - 2018/1/12

Y1 - 2018/1/12

N2 - Objective: Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. Data description: We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion. No evidence was acquired to support idea of multiple T-DNA insertions in the N. glauca genome. Our data is the first comprehensive de novo assembly of tree tobacco and provide valuable information for researches in pharmacological and in phylogenetic fields.

AB - Objective: Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. Data description: We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion. No evidence was acquired to support idea of multiple T-DNA insertions in the N. glauca genome. Our data is the first comprehensive de novo assembly of tree tobacco and provide valuable information for researches in pharmacological and in phylogenetic fields.

KW - Cellular T-DNA

KW - Genome assembly

KW - Nicotiana glauca

KW - Whole genome sequencing

KW - High-Throughput Nucleotide Sequencing/methods

KW - Tobacco/genetics

KW - Gene Transfer, Horizontal/genetics

KW - DNA, Bacterial/genetics

KW - Genome, Plant/genetics

KW - Sequence Analysis, DNA/methods

UR - http://www.scopus.com/inward/record.url?scp=85040446726&partnerID=8YFLogxK

UR - https://doi.org/10.1186%2Fs13104-018-3127-x

UR - http://www.mendeley.com/research/nicotiana-glauca-wholegenome-investigation-ctdna-study

U2 - 10.1101/211482

DO - 10.1101/211482

M3 - Article

C2 - 29329601

VL - 11

JO - BMC Research Notes

JF - BMC Research Notes

SN - 1756-0500

IS - 1

M1 - 18

ER -

ID: 72771684