Research output: Contribution to journal › Article › peer-review
Nicotiana glauca whole-genome investigation for cT-DNA study. / Хафизова, Галина Васильевна; Добрынин, Павел Владимирович; Полев, Дмитрий Евгеньевич; Матвеева, Татьяна Валерьевна.
In: BMC Research Notes, Vol. 11, No. 1, 18, 12.01.2018.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Nicotiana glauca whole-genome investigation for cT-DNA study
AU - Хафизова, Галина Васильевна
AU - Добрынин, Павел Владимирович
AU - Полев, Дмитрий Евгеньевич
AU - Матвеева, Татьяна Валерьевна
N1 - Funding Information: This paper was supported by a Grant to Tatiana Matveeva from the Russian Science Foundation 16-16-10010 and a Grant from Saint Petersburg State University №1.52.1647.2016.
PY - 2018/1/12
Y1 - 2018/1/12
N2 - Objective: Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. Data description: We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion. No evidence was acquired to support idea of multiple T-DNA insertions in the N. glauca genome. Our data is the first comprehensive de novo assembly of tree tobacco and provide valuable information for researches in pharmacological and in phylogenetic fields.
AB - Objective: Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. Data description: We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion. No evidence was acquired to support idea of multiple T-DNA insertions in the N. glauca genome. Our data is the first comprehensive de novo assembly of tree tobacco and provide valuable information for researches in pharmacological and in phylogenetic fields.
KW - Cellular T-DNA
KW - Genome assembly
KW - Nicotiana glauca
KW - Whole genome sequencing
KW - High-Throughput Nucleotide Sequencing/methods
KW - Tobacco/genetics
KW - Gene Transfer, Horizontal/genetics
KW - DNA, Bacterial/genetics
KW - Genome, Plant/genetics
KW - Sequence Analysis, DNA/methods
UR - http://www.scopus.com/inward/record.url?scp=85040446726&partnerID=8YFLogxK
UR - https://doi.org/10.1186%2Fs13104-018-3127-x
UR - http://www.mendeley.com/research/nicotiana-glauca-wholegenome-investigation-ctdna-study
U2 - 10.1101/211482
DO - 10.1101/211482
M3 - Article
C2 - 29329601
VL - 11
JO - BMC Research Notes
JF - BMC Research Notes
SN - 1756-0500
IS - 1
M1 - 18
ER -
ID: 72771684