The species Leptomyxa neglecta (Smirnov, Nassonova, Fahrni and Pawlowski, 2009) Smirnov et al., 2017 (Amoebozoa, Tubulinea, Leptomyxida) was described from the bottom sediment of the Lake Leshevoe (Valamo Island, Northwestern Russia), based on the material collected in the year 1998. The description, published in 2009, was illustrated with phase-contrast microphotographs made by film camera. In 2019, we re-isolated this species from the Lake Nikonovskoe (Valamo Island), located in one kilometer from the type habitat. The amoebae of the studied strain called LVal were identified as L. neglecta by the congruence of basic morphological characters and complete sequence identity of the 18S rRNA gene sequence. We obtained high-quality DIC and IMC images of this species. New data on the possible shapes of the floating cell, morphology and behavior of flattened amoeba and on the structure of the nucleolus are presented. Our study shows that L. neglecta is capable of locomotion in a flabellate form and fusion of pseudopodia, but without the formation of fenestrated, reticulate cells.