The lichen biota of the Amur Region (southern Russian Far East) was studied in the course of several geobotanical expeditions. In total 38 species of lichens and three lichenicolous fungi are reported for the first time for the Amur Region. Among them four species (Fulgidea oligospora, Peltigera frippii, Psora globifera, and Rhizoplaca subdiscrepans) are new to the Russian Far East and nine more — to the southern Russian Far East. Four and 38 species are new to the Zeya Reserve and Tokinsko-Stanovoy National Park, respectively.