New Approach to the Formation of Physically Adsorbed Capillary Coatings Consisting of Hyperbranched Poly(Ethylene Imine) with a Maltose Shell to Enhance the Separation of Catecholamines and Proteins in CE

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New Approach to the Formation of Physically Adsorbed Capillary Coatings Consisting of Hyperbranched Poly(Ethylene Imine) with a Maltose Shell to Enhance the Separation of Catecholamines and Proteins in CE. / Dzema, Daria; Kartsova, Liudmila; Kapizova, Diana; Appelhans, Dietmar.

In: Chromatographia, Vol. 80, No. 11, 01.11.2017, p. 1683-1693.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{e53b8dff947a43e396619fd10fa2fb51,

title = "New Approach to the Formation of Physically Adsorbed Capillary Coatings Consisting of Hyperbranched Poly(Ethylene Imine) with a Maltose Shell to Enhance the Separation of Catecholamines and Proteins in CE",

abstract = "Core–shell-type polymers based on a hyperbranched (hb) poly(ethylenimine) core and a shell with a variable maltose content were applied as coating materials for fused silica capillaries. A new, simple, fast, and reproducible way of modifying the capillary walls through the physical adsorption of the core–shell-type polymers using a Cu2+ support was developed. The coating created by this method was found to be very stable compared to the coating created using a solution of the polymer only. Capillaries modified with the core–shell-type polymers were tested by applying them to the electrophoretic separation of catecholamines and proteins. The modified capillaries showed high efficiencies (up to 800,000 theoretical plates per meter for lysozyme) and separation selectivities. The highest efficiency was achieved using capillaries modified with the polymer containing the lowest content of maltose in the shell and the most accessible positively charged core. Various online concentration techniques were also tested as a means to lower detection limits further, making it possible to analyze proteins in biological fluids (saliva) as well as catecholamines in human urine after SPE using activated alumina.",

keywords = "Capillary electrophoresis, Catecholamines, Coating materials, Hyperbranched polymers, Proteins",

author = "Daria Dzema and Liudmila Kartsova and Diana Kapizova and Dietmar Appelhans",

year = "2017",

month = nov,

day = "1",

doi = "10.1007/s10337-017-3390-3",

language = "English",

volume = "80",

pages = "1683--1693",

journal = "Chromatographia",

issn = "0009-5893",

publisher = "Springer Nature",

number = "11",

}

RIS

TY - JOUR

T1 - New Approach to the Formation of Physically Adsorbed Capillary Coatings Consisting of Hyperbranched Poly(Ethylene Imine) with a Maltose Shell to Enhance the Separation of Catecholamines and Proteins in CE

AU - Dzema, Daria

AU - Kartsova, Liudmila

AU - Kapizova, Diana

AU - Appelhans, Dietmar

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Core–shell-type polymers based on a hyperbranched (hb) poly(ethylenimine) core and a shell with a variable maltose content were applied as coating materials for fused silica capillaries. A new, simple, fast, and reproducible way of modifying the capillary walls through the physical adsorption of the core–shell-type polymers using a Cu2+ support was developed. The coating created by this method was found to be very stable compared to the coating created using a solution of the polymer only. Capillaries modified with the core–shell-type polymers were tested by applying them to the electrophoretic separation of catecholamines and proteins. The modified capillaries showed high efficiencies (up to 800,000 theoretical plates per meter for lysozyme) and separation selectivities. The highest efficiency was achieved using capillaries modified with the polymer containing the lowest content of maltose in the shell and the most accessible positively charged core. Various online concentration techniques were also tested as a means to lower detection limits further, making it possible to analyze proteins in biological fluids (saliva) as well as catecholamines in human urine after SPE using activated alumina.

AB - Core–shell-type polymers based on a hyperbranched (hb) poly(ethylenimine) core and a shell with a variable maltose content were applied as coating materials for fused silica capillaries. A new, simple, fast, and reproducible way of modifying the capillary walls through the physical adsorption of the core–shell-type polymers using a Cu2+ support was developed. The coating created by this method was found to be very stable compared to the coating created using a solution of the polymer only. Capillaries modified with the core–shell-type polymers were tested by applying them to the electrophoretic separation of catecholamines and proteins. The modified capillaries showed high efficiencies (up to 800,000 theoretical plates per meter for lysozyme) and separation selectivities. The highest efficiency was achieved using capillaries modified with the polymer containing the lowest content of maltose in the shell and the most accessible positively charged core. Various online concentration techniques were also tested as a means to lower detection limits further, making it possible to analyze proteins in biological fluids (saliva) as well as catecholamines in human urine after SPE using activated alumina.

KW - Capillary electrophoresis

KW - Catecholamines

KW - Coating materials

KW - Hyperbranched polymers

KW - Proteins

UR - http://www.scopus.com/inward/record.url?scp=85028982726&partnerID=8YFLogxK

U2 - 10.1007/s10337-017-3390-3

DO - 10.1007/s10337-017-3390-3

M3 - Article

AN - SCOPUS:85028982726

VL - 80

SP - 1683

EP - 1693

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

IS - 11

ER -

ID: 40041686