Neurulation in the posterior region of zebrafish, Danio rerio embryos

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Neurulation in the posterior region of zebrafish, Danio rerio embryos. / Gladysheva, Julia; Evnukova, Evdokia; Kondakova, Ekaterina ; Kulakova, Milana ; Efremov, Vladimir.

In: Journal of Morphology, Vol. 282, No. 10, 10.2021, p. 1437-1454.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{c4cab1cc65d4421eac26ee6bb8fb42af,

title = "Neurulation in the posterior region of zebrafish, Danio rerio embryos",

abstract = "The neural tube of amniotes is formed through different mechanisms that take place in the anterior and posterior regions and involve neural plate folding or mesenchymal condensation followed by its cavitation. Meanwhile, in teleost trunk region, the neural plate forms the neural keel, while the lumen develops later. However, the data on neurulation and other morphogenetic processes in the posterior body region in Teleostei remain fragmentary. We proposed that there could be variations in the morphogenetic processes, such as cell shape changes and cell rearrangements, in the posterior region compared to the anterior one at the different stages. Here, we performed morphological and histochemical analyses of morphogenetic processes with an emphasis on neurulation in the zebrafish tail bud (TB) and posterior region. To analyze the posterior expression of sox2 and tbxta we performed whole mount in situ hybridization. We showed that the TB cells of variable shapes and orientation are tightly packed, and the neural and notochord primordia develop first. The shape of the neural primordium undergoes numerous changes as a result of cell rearrangements leading to the development of the neural rod. At the prim-6 stage, the cells of the neural primordium directly form the neural rod. The neuroepithelial cells undergo sequential shape changes. At the stage of the neural rod formation, the apical regions of triangular neuroepithelial cells of the floor plate are enriched in F-actin. The neurocoel development onset is above the apical poles of neuroepithelial cells. The expression domains of sox2 and tbxta become more restricted during the development.",

keywords = "f-actin, notochord, sox2, tail bud, tbxta, TAIL BUD, NEURAL-TUBE CLOSURE, SOX2, XENOPUS-LAEVIS, CELL-DIVISION, MORPHOGENESIS, SHAPE, SECONDARY NEURULATION, APICAL CONSTRICTION, EXPRESSION",

author = "Julia Gladysheva and Evdokia Evnukova and Ekaterina Kondakova and Milana Kulakova and Vladimir Efremov",

note = "Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC.",

year = "2021",

month = oct,

doi = "10.1002/jmor.21396",

language = "English",

volume = "282",

pages = "1437--1454",

journal = "Journal of Morphology",

issn = "0362-2525",

publisher = "Wiley-Blackwell",

number = "10",

}

RIS

TY - JOUR

T1 - Neurulation in the posterior region of zebrafish, Danio rerio embryos

AU - Gladysheva, Julia

AU - Evnukova, Evdokia

AU - Kondakova, Ekaterina

AU - Kulakova, Milana

AU - Efremov, Vladimir

PY - 2021/10

Y1 - 2021/10

N2 - The neural tube of amniotes is formed through different mechanisms that take place in the anterior and posterior regions and involve neural plate folding or mesenchymal condensation followed by its cavitation. Meanwhile, in teleost trunk region, the neural plate forms the neural keel, while the lumen develops later. However, the data on neurulation and other morphogenetic processes in the posterior body region in Teleostei remain fragmentary. We proposed that there could be variations in the morphogenetic processes, such as cell shape changes and cell rearrangements, in the posterior region compared to the anterior one at the different stages. Here, we performed morphological and histochemical analyses of morphogenetic processes with an emphasis on neurulation in the zebrafish tail bud (TB) and posterior region. To analyze the posterior expression of sox2 and tbxta we performed whole mount in situ hybridization. We showed that the TB cells of variable shapes and orientation are tightly packed, and the neural and notochord primordia develop first. The shape of the neural primordium undergoes numerous changes as a result of cell rearrangements leading to the development of the neural rod. At the prim-6 stage, the cells of the neural primordium directly form the neural rod. The neuroepithelial cells undergo sequential shape changes. At the stage of the neural rod formation, the apical regions of triangular neuroepithelial cells of the floor plate are enriched in F-actin. The neurocoel development onset is above the apical poles of neuroepithelial cells. The expression domains of sox2 and tbxta become more restricted during the development.

AB - The neural tube of amniotes is formed through different mechanisms that take place in the anterior and posterior regions and involve neural plate folding or mesenchymal condensation followed by its cavitation. Meanwhile, in teleost trunk region, the neural plate forms the neural keel, while the lumen develops later. However, the data on neurulation and other morphogenetic processes in the posterior body region in Teleostei remain fragmentary. We proposed that there could be variations in the morphogenetic processes, such as cell shape changes and cell rearrangements, in the posterior region compared to the anterior one at the different stages. Here, we performed morphological and histochemical analyses of morphogenetic processes with an emphasis on neurulation in the zebrafish tail bud (TB) and posterior region. To analyze the posterior expression of sox2 and tbxta we performed whole mount in situ hybridization. We showed that the TB cells of variable shapes and orientation are tightly packed, and the neural and notochord primordia develop first. The shape of the neural primordium undergoes numerous changes as a result of cell rearrangements leading to the development of the neural rod. At the prim-6 stage, the cells of the neural primordium directly form the neural rod. The neuroepithelial cells undergo sequential shape changes. At the stage of the neural rod formation, the apical regions of triangular neuroepithelial cells of the floor plate are enriched in F-actin. The neurocoel development onset is above the apical poles of neuroepithelial cells. The expression domains of sox2 and tbxta become more restricted during the development.

KW - f-actin

KW - notochord

KW - sox2

KW - tail bud

KW - tbxta

KW - TAIL BUD

KW - NEURAL-TUBE CLOSURE

KW - SOX2

KW - XENOPUS-LAEVIS

KW - CELL-DIVISION

KW - MORPHOGENESIS

KW - SHAPE

KW - SECONDARY NEURULATION

KW - APICAL CONSTRICTION

KW - EXPRESSION

UR - http://www.scopus.com/inward/record.url?scp=85109700158&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/1e2bb28d-4875-3b03-97a0-d69786b4a98f/

U2 - 10.1002/jmor.21396

DO - 10.1002/jmor.21396

M3 - Article

AN - SCOPUS:85109700158

VL - 282

SP - 1437

EP - 1454

JO - Journal of Morphology

JF - Journal of Morphology

SN - 0362-2525

IS - 10

ER -

ID: 84325649