TY - JOUR

T1 - Nanostructure of bone tissue probed with Ca 2p and O 1s NEXAFS spectroscopy

AU - Сахоненков, Сергей Сергеевич

AU - Конашук, Алексей Сергеевич

AU - Брыкалова, Ксения Олеговна

AU - Черный, А. А.

AU - Риков, Ю. А.

AU - Корнилов, Н. Н.

AU - Филатова, Елена Олеговна

AU - Павлычев, Андрей Алексеевич

PY - 2021/6/1

Y1 - 2021/6/1

N2 - X-ray absorption spectroscopy is applied to investigate relationships between hierarchical organization of the skeleton and nanostructure of femoral bone in knee compartments and to understand the osteoarthritis (OA) related changes at the subcellular level. Our focus is on local electronic and atomic and molecular architectonics of the medial and lateral condyles of the femur resected during total knee arthroplasty in patients with medial compartmental knee OA. The element-specific and sitedependent peculiarities in spectral distributions of oscillator strength for core-to-valence transitions are revealed. The near Ca 2p andO1s edges x-ray absorption fine structure (Ca 2p andO1s NEXAFS) spectra of the saw cuts demonstrate substantial redistributions in intact and OA damaged areas on the proximal side, and on the proximal and distal sides of the samples. Examining the O1s NEXAFS spectra new chemical bonds are revealed on the proximal surface in the OA areas. Strong intra-atomic intershell Ca2+ 2p(3/2)(,1/2)(5) 3d(1) interaction specifies the great similarity of the Ca 2p NEXAFS spectra. Their analysis performed in combination with the x-ray photoelectron data has demonstrated the formation of non-apatite calcium in the OA areas of the samples. It is shown that NEXAFS spectroscopy is a powerful tool for deeper understanding relationship between hierarchical skeletal organization and nanostructure of native bone. Perspectives for development of novel methods for medical imaging and diagnosis of subchondral bone at the nanolevel are discussed.

AB - X-ray absorption spectroscopy is applied to investigate relationships between hierarchical organization of the skeleton and nanostructure of femoral bone in knee compartments and to understand the osteoarthritis (OA) related changes at the subcellular level. Our focus is on local electronic and atomic and molecular architectonics of the medial and lateral condyles of the femur resected during total knee arthroplasty in patients with medial compartmental knee OA. The element-specific and sitedependent peculiarities in spectral distributions of oscillator strength for core-to-valence transitions are revealed. The near Ca 2p andO1s edges x-ray absorption fine structure (Ca 2p andO1s NEXAFS) spectra of the saw cuts demonstrate substantial redistributions in intact and OA damaged areas on the proximal side, and on the proximal and distal sides of the samples. Examining the O1s NEXAFS spectra new chemical bonds are revealed on the proximal surface in the OA areas. Strong intra-atomic intershell Ca2+ 2p(3/2)(,1/2)(5) 3d(1) interaction specifies the great similarity of the Ca 2p NEXAFS spectra. Their analysis performed in combination with the x-ray photoelectron data has demonstrated the formation of non-apatite calcium in the OA areas of the samples. It is shown that NEXAFS spectroscopy is a powerful tool for deeper understanding relationship between hierarchical skeletal organization and nanostructure of native bone. Perspectives for development of novel methods for medical imaging and diagnosis of subchondral bone at the nanolevel are discussed.

KW - nanostructure

KW - bone tissue

KW - Ca 2p andO1s NEXAFS spectra

KW - hierarchical matter

KW - subcellular diagnostic of bone

KW - electronic and atomic structure

KW - INNER-SHELL EXCITATION

KW - RAY-ABSORPTION SPECTRA

KW - LIQUID WATER

KW - YIELD

KW - ORIENTATION

KW - COLLAGEN

UR - https://www.mendeley.com/catalogue/9766dee8-a7a7-3c25-97be-214ea5e376ff/

U2 - 10.1088/2632-959X/abf3a5

DO - 10.1088/2632-959X/abf3a5

M3 - Article

VL - 2

SP - 1

EP - 8

JO - Nano Express

JF - Nano Express

SN - 2632-959X

IS - 2

M1 - 020009

ER -