Research output: Contribution to journal › Article › peer-review
Nanosized cation exchanger for the electrophoretic separation and preconcentration of catecholamines and amino acids. / Polikarpova, Daria; Makeeva, Daria; Kolotilina, Nadezhda; Dolgonosov, Anatoly; Peshkova, Maria; Kartsova, Liudmila.
In: Electrophoresis, Vol. 41, No. 12, 01.06.2020, p. 1031-1038.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Nanosized cation exchanger for the electrophoretic separation and preconcentration of catecholamines and amino acids
AU - Polikarpova, Daria
AU - Makeeva, Daria
AU - Kolotilina, Nadezhda
AU - Dolgonosov, Anatoly
AU - Peshkova, Maria
AU - Kartsova, Liudmila
PY - 2020/6/1
Y1 - 2020/6/1
N2 - The first example of application of nanosized polystyrene-based cation exchanger (NSCE) with sulfo groups as a dynamic coating of capillary walls was demonstrated. The conditions of dynamic coating formation were optimized and ensured the long-term stability of the coating. Capillary-to-capillary and day-to-day repeatabilities were 4% and 3%, correspondingly. The NSCE coating stability at various pH and influence of pH on the EOF mobility were investigated. The developed NSCE-modified coated capillaries provided improved resolution (Rs = 0.9–3.2 for catecholamines and Rs = 1.7–2.8 for amino acids) and efficiencies (330–520 ×103 t.p./m) of basic analytes, which are 1.5 times higher compared to untreated capillary. The optimized conditions were as follows: 50 mM phosphate buffer solution at pH 2.2 with 5 μM NSCE. The effect of the NSCE concentration in BGE on the electrophoretic mobilities of the analytes was investigated. The various online concentration techniques were tested in order to decrease the LODs. The simultaneous application of NSCE capillaries and field-amplified sample stacking provided the lowest LODs of catecholamines and amino acids and allowed to determine these analytes in human urine.
AB - The first example of application of nanosized polystyrene-based cation exchanger (NSCE) with sulfo groups as a dynamic coating of capillary walls was demonstrated. The conditions of dynamic coating formation were optimized and ensured the long-term stability of the coating. Capillary-to-capillary and day-to-day repeatabilities were 4% and 3%, correspondingly. The NSCE coating stability at various pH and influence of pH on the EOF mobility were investigated. The developed NSCE-modified coated capillaries provided improved resolution (Rs = 0.9–3.2 for catecholamines and Rs = 1.7–2.8 for amino acids) and efficiencies (330–520 ×103 t.p./m) of basic analytes, which are 1.5 times higher compared to untreated capillary. The optimized conditions were as follows: 50 mM phosphate buffer solution at pH 2.2 with 5 μM NSCE. The effect of the NSCE concentration in BGE on the electrophoretic mobilities of the analytes was investigated. The various online concentration techniques were tested in order to decrease the LODs. The simultaneous application of NSCE capillaries and field-amplified sample stacking provided the lowest LODs of catecholamines and amino acids and allowed to determine these analytes in human urine.
KW - Amino acids
KW - Catecholamines
KW - CE
KW - Nanosized cation exchanger
KW - Online concentration
KW - COATED CAPILLARY
KW - CAPILLARY ELECTROCHROMATOGRAPHIC SEPARATION
KW - COLUMN
KW - IONIC LIQUIDS
KW - ONLINE CONCENTRATION
UR - http://www.scopus.com/inward/record.url?scp=85084728785&partnerID=8YFLogxK
U2 - 10.1002/elps.201900416
DO - 10.1002/elps.201900416
M3 - Article
C2 - 32176938
AN - SCOPUS:85084728785
VL - 41
SP - 1031
EP - 1038
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 12
ER -
ID: 61636171