Research output: Contribution to journal › Article › peer-review
Nano-sized anion-exchangers (NSAE) are promising materials in electrophoretic separation methods due to their high ion-exchange capacity, large surface-to-volume ratios, high adhesion to the quartz surface and pH-independent positive charge. In current research we describe a simple approach for NSAE synthesis, which includes two-step grinding of macroanionite followed by centrifugation. The synthesized stable aqueous suspension of NSAE particles was applied as physically adsorbed modifier of fused-silica capillary walls for CEC separation of carboxylic acids. We proposed fast and simple approach to formation of NSAE-based stationary phase on the internal fused-silica surface, which included 15 min rinsing of the capillary with diluted water suspension of NSAE. Formed physically-adsorbed coating turned out to be extremely stable in a wide range of pH (from 2 to 10). NSAE modified capillaries provided high separation efficiency (N = 148–732 *103 t.p./m) and selectivity (Rs = 1.2–5.7) of carboxylic acids. Simultaneous application of NSAE-modified capillaries with various on-line concentration techniques (such as field amplified sample stacking and field amplified sample injection) provided both low detection limits (up to 1–3 ng/mL) and high separation selectivity of carboxylic acids. It was useful for their quantitative determination in wines samples. Physically-adsorbed coatings based on NSAE exhibit higher selectivity and lower detection limits compared to commonly used dynamic modifier of fused-silica capillary walls - cetyltrimethylammonium bromide. NSAE-based coatings do not require equilibrium sustaining to maintain the surface coverage. It makes them appropriate for CE-MS application.
Original language | English |
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Pages (from-to) | 744-749 |
Number of pages | 6 |
Journal | Talanta |
Volume | 188 |
DOIs | |
State | Published - 1 Oct 2018 |
ID: 40041225