The algorithm for interpreting ground-based measurements of the brightness temperature of downwelling microwave radiation in absorption lines traditionally used for to retrieve ozone vertical profiles is described. The inverse problem is formulated as a multiparameter with respect to ozone vertical profile (target parameter), as well as vertical profiles of the temperature, pressure, water vapor content, and cloud liquid water (controlled parameters). This approach allows one to use independent measurements of atmospheric parameters in different altitude ranges (if available) in any combinations and accounting for physical relationships between the parameters (e.g., the hydrostatic equation for temperature and pressure). Error estimates for retrieving the mean ozone concentration in the 22–30-, 30–40-, 40–50-, and 50–60-km layers are presented for summer and winter conditions (for different tropospheric total water content) and different scenarios of interpreting the downwelling microwave radiation measurements in the 110-GHz ozone absorption line.