Research output: Contribution to journal › Article › peer-review
Multinucleated Cells Resistant to Genotoxic Factors within Human Glioblastoma Cell Lines. / Kiseleva, L. N.; Kartashev, A. V.; Vartanyan, N. L.; Pinevich, A. A.; Samoilovich, M. P.
In: Cell and Tissue Biology, Vol. 13, No. 1, 01.01.2019.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Multinucleated Cells Resistant to Genotoxic Factors within Human Glioblastoma Cell Lines
AU - Kiseleva, L. N.
AU - Kartashev, A. V.
AU - Vartanyan, N. L.
AU - Pinevich, A. A.
AU - Samoilovich, M. P.
N1 - Publisher Copyright: © 2019, Pleiades Publishing, Ltd. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Abstract: Glioblastoma tumors are characterized by a high phenotype diversity among patients and by a high cellular heterogeneity within each tumor instance. Here, we report the effects of photon irradiation of various dosage and regime onto cells of two reference (T98G and A172) and two novel (T2 and R1) human glioblastoma cell lines. In contrast to the A172 and R1 lines, where all cells were perished upon relatively low-dose irradiation, a population of resistant cells within the T98G and T2 lines survived even after a high-dose irradiation. These radio-resistant cells were large single- or multinucleated non-proliferating cells, that remained viable over one month after irradiation. The cells had markedly elevated expression levels of genes coding for factors promoting growth and angiogenesis, components of the extracellular matrix, and marker genes in-herent for mesenchymal phenotype. The expression profile of these genes in the radiation-induced growth-arrested cells T98G and Т2 was similar to that of the cell lines treated with another genotoxic factor, the fotemustine. Therefore, radio-resistant growth-arrested cells are characterized by a high activity of genes encoding proteins affecting both the tumor cells and their microenvironment.
AB - Abstract: Glioblastoma tumors are characterized by a high phenotype diversity among patients and by a high cellular heterogeneity within each tumor instance. Here, we report the effects of photon irradiation of various dosage and regime onto cells of two reference (T98G and A172) and two novel (T2 and R1) human glioblastoma cell lines. In contrast to the A172 and R1 lines, where all cells were perished upon relatively low-dose irradiation, a population of resistant cells within the T98G and T2 lines survived even after a high-dose irradiation. These radio-resistant cells were large single- or multinucleated non-proliferating cells, that remained viable over one month after irradiation. The cells had markedly elevated expression levels of genes coding for factors promoting growth and angiogenesis, components of the extracellular matrix, and marker genes in-herent for mesenchymal phenotype. The expression profile of these genes in the radiation-induced growth-arrested cells T98G and Т2 was similar to that of the cell lines treated with another genotoxic factor, the fotemustine. Therefore, radio-resistant growth-arrested cells are characterized by a high activity of genes encoding proteins affecting both the tumor cells and their microenvironment.
KW - extracellular matrix proteins
KW - fotemustine
KW - genotoxic factors
KW - glioblastoma
KW - growth factor genes
KW - growth-arrested cells
KW - irradiation
KW - irradiation-resistant cells
KW - mesenchymal markers
KW - multinucleated cells
KW - R1
KW - А172
KW - Т2
KW - Т98G
UR - http://www.scopus.com/inward/record.url?scp=85065665529&partnerID=8YFLogxK
U2 - 10.1134/S1990519X19010061
DO - 10.1134/S1990519X19010061
M3 - Article
AN - SCOPUS:85065665529
VL - 13
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 1
ER -
ID: 76654648