Multifunction covalent coatings for separation of amino acids, biogenic amines, steroid hormones, and ketoprofen enantiomers by capillary electrophoresis and capillary electrochromatography

Standard

Multifunction covalent coatings for separation of amino acids, biogenic amines, steroid hormones, and ketoprofen enantiomers by capillary electrophoresis and capillary electrochromatography. / Kravchenko, Anastasia ; Kolobova, Ekaterina ; Kartsova, Liudmila .

In: Separation Science Plus, Vol. 3, No. 4, 04.2020, p. 102-111.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{af31ba2870a548c99148a2201cd06af7,

title = "Multifunction covalent coatings for separation of amino acids, biogenic amines, steroid hormones, and ketoprofen enantiomers by capillary electrophoresis and capillary electrochromatography",

abstract = "In this study, the preparation process of multifunctional coating based on imidazole and beta-cyclodextrin has been developed. The synthesis includes several steps: activation of fused silica capillary walls via alkali, then process of silylation and finally functionalization with imidazole and beta-cyclodextrin derivative. The latter step was carried over 22 h at 100 degrees C at 750 mg/mL concentration of p-toluenesulfonyl-beta-cyclodextrin. It was established that the coatings thus obtained are stable within pH 2-7 range but decompose in alkaline medium. The stationary phase developed in this study allows simultaneous separation of hydrophobic and hydrophilic analytes. Chiral separation of ketoprofen enantiomers was also achieved with the newly developed covalent coating. It was found that covalent coating based on imidazole and beta-cyclodextrin exceeded the dynamic coatings of similar structure in terms of repeatability, analysis time and the limits of detection.",

keywords = "BACKGROUND ELECTROLYTE, BETA-CYCLODEXTRINS, Capillary electrochromatography, Chiral selectors, Covalent coatings, Cyclodextrin, ELECTROKINETIC CHROMATOGRAPHY, ELECTROMIGRATION METHODS, ENANTIOSEPARATION, Imidazolium ionic liquids, MODIFIED SILICA, PERFORMANCE, STATIONARY-PHASE, TEMPERATURE IONIC LIQUIDS, ZONE-ELECTROPHORESIS",

author = "Anastasia Kravchenko and Ekaterina Kolobova and Liudmila Kartsova",

year = "2020",

month = apr,

doi = "DOI: 10.1002/sscp.201900098",

language = "English",

volume = "3",

pages = "102--111",

journal = "Separation Science Plus",

issn = "2573-1815",

publisher = "Wiley-Blackwell",

number = "4",

}

RIS

TY - JOUR

T1 - Multifunction covalent coatings for separation of amino acids, biogenic amines, steroid hormones, and ketoprofen enantiomers by capillary electrophoresis and capillary electrochromatography

AU - Kravchenko, Anastasia

AU - Kolobova, Ekaterina

AU - Kartsova, Liudmila

PY - 2020/4

Y1 - 2020/4

N2 - In this study, the preparation process of multifunctional coating based on imidazole and beta-cyclodextrin has been developed. The synthesis includes several steps: activation of fused silica capillary walls via alkali, then process of silylation and finally functionalization with imidazole and beta-cyclodextrin derivative. The latter step was carried over 22 h at 100 degrees C at 750 mg/mL concentration of p-toluenesulfonyl-beta-cyclodextrin. It was established that the coatings thus obtained are stable within pH 2-7 range but decompose in alkaline medium. The stationary phase developed in this study allows simultaneous separation of hydrophobic and hydrophilic analytes. Chiral separation of ketoprofen enantiomers was also achieved with the newly developed covalent coating. It was found that covalent coating based on imidazole and beta-cyclodextrin exceeded the dynamic coatings of similar structure in terms of repeatability, analysis time and the limits of detection.

AB - In this study, the preparation process of multifunctional coating based on imidazole and beta-cyclodextrin has been developed. The synthesis includes several steps: activation of fused silica capillary walls via alkali, then process of silylation and finally functionalization with imidazole and beta-cyclodextrin derivative. The latter step was carried over 22 h at 100 degrees C at 750 mg/mL concentration of p-toluenesulfonyl-beta-cyclodextrin. It was established that the coatings thus obtained are stable within pH 2-7 range but decompose in alkaline medium. The stationary phase developed in this study allows simultaneous separation of hydrophobic and hydrophilic analytes. Chiral separation of ketoprofen enantiomers was also achieved with the newly developed covalent coating. It was found that covalent coating based on imidazole and beta-cyclodextrin exceeded the dynamic coatings of similar structure in terms of repeatability, analysis time and the limits of detection.

KW - BACKGROUND ELECTROLYTE

KW - BETA-CYCLODEXTRINS

KW - Capillary electrochromatography

KW - Chiral selectors

KW - Covalent coatings

KW - Cyclodextrin

KW - ELECTROKINETIC CHROMATOGRAPHY

KW - ELECTROMIGRATION METHODS

KW - ENANTIOSEPARATION

KW - Imidazolium ionic liquids

KW - MODIFIED SILICA

KW - PERFORMANCE

KW - STATIONARY-PHASE

KW - TEMPERATURE IONIC LIQUIDS

KW - ZONE-ELECTROPHORESIS

UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/sscp.201900098

U2 - DOI: 10.1002/sscp.201900098

DO - DOI: 10.1002/sscp.201900098

M3 - Article

VL - 3

SP - 102

EP - 111

JO - Separation Science Plus

JF - Separation Science Plus

SN - 2573-1815

IS - 4

ER -

ID: 52420987