The multi-analytical study of the XIII - XIV century French medieval Legendarium manuscript is presented. In the beginning of the XX century in Petrograd-Leningrad the manuscript was seriously damaged. The parchment has become moldy and rotten because it was soaked in water for unknown reasons, and then kept in a closed safe. The sheets of parchment after drying stuck together in integral block. In the 1960-s, despite the depressing state of the parchment “brick”, it was decided to start a restoration work on the separation of glued sheets. Now the manuscript consists of 214 sheets encapsulated in polyethylene terephthalate (PET) conservation envelopes. The damaged manuscript sheets are in different states, some illustrations and text parts are in rather bad condition. At the same time, the blurred traces of pigments and inks, which were used to write letters and make illustrations, were preserved on the parchment. Works carried out with this unique monument, include research using the optical microscopy (OM), micro-Raman spectroscopy, shifted- excitation Raman difference spectroscopy (SERDS) technique and Scanning Electron Microscopy (SEM) with EDX analysis. Moreover, the research focused on micro-particles, which were found inside the envelopes and were collected by restorers. The conducted study allow to judge about the composition of the ink and pigments which were used to create the manuscript. Non-destructive research opens up perspectives for developing the new ways for the possible reconstruction of the lost text elements and codex images.