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MtCLE08, MtCLE16, and MtCLE18 Transcription Patterns and Their Possible Functions in the Embryogenic Calli of Medicago truncatula. / Кудряшов, Андрей Александрович; Злыднева, Наталья Сергеевна; Ефремова, Елена Павловна; Творогова, Варвара Евгеньевна; Лутова, Людмила Алексеевна.

In: Plants, Vol. 12, No. 3, 435, 17.01.2023.

Research output: Contribution to journal › Article › peer-review

Harvard

Кудряшов, АА, Злыднева, НС, Ефремова, ЕП, Творогова, ВЕ & Лутова, ЛА 2023, 'MtCLE08, MtCLE16, and MtCLE18 Transcription Patterns and Their Possible Functions in the Embryogenic Calli of Medicago truncatula', Plants, vol. 12, no. 3, 435. https://doi.org/10.3390/plants12030435

APA

Кудряшов, А. А., Злыднева, Н. С., Ефремова, Е. П., Творогова, В. Е., & Лутова, Л. А. (2023). MtCLE08, MtCLE16, and MtCLE18 Transcription Patterns and Their Possible Functions in the Embryogenic Calli of Medicago truncatula. Plants, 12(3), [435]. https://doi.org/10.3390/plants12030435

Vancouver

Кудряшов АА, Злыднева НС, Ефремова ЕП, Творогова ВЕ, Лутова ЛА. MtCLE08, MtCLE16, and MtCLE18 Transcription Patterns and Their Possible Functions in the Embryogenic Calli of Medicago truncatula. Plants. 2023 Jan 17;12(3). 435. https://doi.org/10.3390/plants12030435

Author

Кудряшов, Андрей Александрович ; Злыднева, Наталья Сергеевна ; Ефремова, Елена Павловна ; Творогова, Варвара Евгеньевна ; Лутова, Людмила Алексеевна. / MtCLE08, MtCLE16, and MtCLE18 Transcription Patterns and Their Possible Functions in the Embryogenic Calli of Medicago truncatula. In: Plants. 2023 ; Vol. 12, No. 3.

BibTeX

@article{fe6982a7f22c4f5ca5e974469b6a4700,
title = "MtCLE08, MtCLE16, and MtCLE18 Transcription Patterns and Their Possible Functions in the Embryogenic Calli of Medicago truncatula",
abstract = "CLE peptides are well-known hormonal regulators of plant development, but their role in somatic embryogenesis remains undetermined. CLE genes are often regulated by WOX transcription factors and, in their turn, regulate the expression level of WOX genes. In this study, we used in vitro cultivation, as well as qPCR and transcriptomic analysis, to find CLE peptides which could regulate the MtWOX9-1 gene, stimulating somatic embryogenesis in Medicago truncatula. Three CLE peptides were found which could probably be such regulators, but none of them was found to influence MtWOX9-1 expression in the embryogenic calli. Nevertheless, overexpression of one of CLE genes under study, MtCLE16, decreased somatic embryogenesis intensity. Additionally, overexpression of MtCLE08 was found to suppress expression of MtWOX13a, a supposed antagonist of somatic embryo development. Our findings can be helpful for the search for new regeneration regulators which could be used for plant transformation. ",
author = "Кудряшов, {Андрей Александрович} and Злыднева, {Наталья Сергеевна} and Ефремова, {Елена Павловна} and Творогова, {Варвара Евгеньевна} and Лутова, {Людмила Алексеевна}",
year = "2023",
month = jan,
day = "17",
doi = "10.3390/plants12030435",
language = "English",
volume = "12",
journal = "Plants",
issn = "2223-7747",
publisher = "MDPI AG",
number = "3",

}

RIS

TY - JOUR

T1 - MtCLE08, MtCLE16, and MtCLE18 Transcription Patterns and Their Possible Functions in the Embryogenic Calli of Medicago truncatula

AU - Кудряшов, Андрей Александрович

AU - Злыднева, Наталья Сергеевна

AU - Ефремова, Елена Павловна

AU - Творогова, Варвара Евгеньевна

AU - Лутова, Людмила Алексеевна

PY - 2023/1/17

Y1 - 2023/1/17

N2 - CLE peptides are well-known hormonal regulators of plant development, but their role in somatic embryogenesis remains undetermined. CLE genes are often regulated by WOX transcription factors and, in their turn, regulate the expression level of WOX genes. In this study, we used in vitro cultivation, as well as qPCR and transcriptomic analysis, to find CLE peptides which could regulate the MtWOX9-1 gene, stimulating somatic embryogenesis in Medicago truncatula. Three CLE peptides were found which could probably be such regulators, but none of them was found to influence MtWOX9-1 expression in the embryogenic calli. Nevertheless, overexpression of one of CLE genes under study, MtCLE16, decreased somatic embryogenesis intensity. Additionally, overexpression of MtCLE08 was found to suppress expression of MtWOX13a, a supposed antagonist of somatic embryo development. Our findings can be helpful for the search for new regeneration regulators which could be used for plant transformation.

AB - CLE peptides are well-known hormonal regulators of plant development, but their role in somatic embryogenesis remains undetermined. CLE genes are often regulated by WOX transcription factors and, in their turn, regulate the expression level of WOX genes. In this study, we used in vitro cultivation, as well as qPCR and transcriptomic analysis, to find CLE peptides which could regulate the MtWOX9-1 gene, stimulating somatic embryogenesis in Medicago truncatula. Three CLE peptides were found which could probably be such regulators, but none of them was found to influence MtWOX9-1 expression in the embryogenic calli. Nevertheless, overexpression of one of CLE genes under study, MtCLE16, decreased somatic embryogenesis intensity. Additionally, overexpression of MtCLE08 was found to suppress expression of MtWOX13a, a supposed antagonist of somatic embryo development. Our findings can be helpful for the search for new regeneration regulators which could be used for plant transformation.

UR - https://www.mendeley.com/catalogue/83b16584-0500-328d-8f62-ad84fc027ce6/

U2 - 10.3390/plants12030435

DO - 10.3390/plants12030435

M3 - Article

C2 - 36771520

VL - 12

JO - Plants

JF - Plants

SN - 2223-7747

IS - 3

M1 - 435

ER -

ID: 102129794