TY - JOUR

T1 - Morphological framework for attachment and locomotion in several Digenea of the families Microphallidae and Heterophyidae

AU - Крупенко, Дарья Юрьевна

AU - Добровольский, Андрей Александрович

N1 - Funding Information: Funding The reported study was funded by Russian Foundation for Basic Research, according to the research project no. 16-34-60156 mol_а_dk, and Saint Petersburg State University project nos. 1.42.1099.2016 and 1.42.739.2017.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Digenea usually use ventral sucker for sustainable attachment within intestine of their definitive vertebrate host. However, if the ventral sucker is absent or poorly developed, the means of attachment are unclear. We investigated attachment and locomotion in such digeneans: three species of the family Microphallidae (Microphallus piriformes, M. pygmaeus, and Levinseniella brachysoma) and two species of the family Heterophyidae (Cryptocotyle concava and C. lingua). Their tegumental spines and musculature were described with use of fluorescent actin staining, confocal microscopy, and scanning electron microscopy. Locomotion of living worms was observed and recorded. Wide serrated tegumental spines probably play the main role in attachment. Their firm contact with the host mucosa may be provided by the action of the ventral concavity—when the entire body or its part acts as a sucker. Dorsoventral muscle bundles act like radial musculature of the sucker generating negative pressure in the ventral concavity. The solid layer of longitudinal muscle fibers on the ventral body surface provides support for the bottom of the ventral concavity. In all microphallids, a U-shaped arrangement of body wall musculature (mostly originating from longitudinal fibers) outlines posterior part of the ventral concavity ridge. In all the studied species, tegumental spines, body wall musculature, and dorsoventral muscle bundles are better developed in the forebody which moves more actively than the hindbody.

AB - Digenea usually use ventral sucker for sustainable attachment within intestine of their definitive vertebrate host. However, if the ventral sucker is absent or poorly developed, the means of attachment are unclear. We investigated attachment and locomotion in such digeneans: three species of the family Microphallidae (Microphallus piriformes, M. pygmaeus, and Levinseniella brachysoma) and two species of the family Heterophyidae (Cryptocotyle concava and C. lingua). Their tegumental spines and musculature were described with use of fluorescent actin staining, confocal microscopy, and scanning electron microscopy. Locomotion of living worms was observed and recorded. Wide serrated tegumental spines probably play the main role in attachment. Their firm contact with the host mucosa may be provided by the action of the ventral concavity—when the entire body or its part acts as a sucker. Dorsoventral muscle bundles act like radial musculature of the sucker generating negative pressure in the ventral concavity. The solid layer of longitudinal muscle fibers on the ventral body surface provides support for the bottom of the ventral concavity. In all microphallids, a U-shaped arrangement of body wall musculature (mostly originating from longitudinal fibers) outlines posterior part of the ventral concavity ridge. In all the studied species, tegumental spines, body wall musculature, and dorsoventral muscle bundles are better developed in the forebody which moves more actively than the hindbody.

KW - Attachment

KW - Digenea

KW - Musculature

KW - Tegumental spines

KW - Ventral concavity

KW - CERCARIAE

KW - METACERCARIAE

KW - ADULTS

KW - NOTOCOTYLIDAE

KW - IDENTIFICATION

KW - MUSCULATURE

UR - http://www.scopus.com/inward/record.url?scp=85053789811&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/morphological-framework-attachment-locomotion-several-digenea-families-microphallidae-heterophyidae

U2 - 10.1007/s00436-018-6085-2

DO - 10.1007/s00436-018-6085-2

M3 - Article

VL - 117

SP - 3799

EP - 3807

JO - Parasitology Research

JF - Parasitology Research

SN - 0932-0113

IS - 12

ER -