Research output: Contribution to journal › Article › peer-review
Monoclonal antibodies against human secretory component: epitope specificity and utility for immunoanalysis. / Griazeva, I. V.; Samoǐlovich, M. P.; Klimovich, V. B.; Pavlova, M. S.; Vartanian, N. L.; Kirienko, A. N.; Klimovich, V. B.
In: Zhurnal mikrobiologii, epidemiologii, i immunobiologii, No. 4, 07.2010, p. 54-59.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Monoclonal antibodies against human secretory component: epitope specificity and utility for immunoanalysis
AU - Griazeva, I. V.
AU - Samoǐlovich, M. P.
AU - Klimovich, V. B.
AU - Pavlova, M. S.
AU - Vartanian, N. L.
AU - Kirienko, A. N.
AU - Klimovich, V. B.
PY - 2010/7
Y1 - 2010/7
N2 - AIM: To develop and characterize by immunochemical methods the panel of monoclonal antibodies (MAbs) recognizing different antigenic determinants ofhuman secretory component (SC) molecule. MATERIALS AND METHODS: sIgA and SC were obtained from colostrum by combination of ion-exchange chromatography and gel filtration. Recombinant SC was expressed in Escherichia coli cells transformed by construction that contained fragment of gene coding extracellular domain of receptor for polymeric Ig. MAbs were produced and studied using hybridoma technologies and different methods of immunoenzyme analysis respectively. RESULTS: Panel comprising 10 MAbs against human SC of which 4 types of antibodies recognize cryptic epitopes of free SC and other 6 types recognize epitopes exposed both on SC and sIgA. MAbs panel contains antibodies interacting with conformational and linear epitopes of antigen. Three from obtained MAbs bind to SC epitopes which structure is determined by presence of carbohydrate residues in the molecule of antigen. Immunometric systems were developed which allow to differentially detect free SC and sIgA. CONCLUSION: Developed and characterized MAbs panel recognizing different epitopes of SC molecule opens new opportunities for laboratory and basic research of human secretory immunity.
AB - AIM: To develop and characterize by immunochemical methods the panel of monoclonal antibodies (MAbs) recognizing different antigenic determinants ofhuman secretory component (SC) molecule. MATERIALS AND METHODS: sIgA and SC were obtained from colostrum by combination of ion-exchange chromatography and gel filtration. Recombinant SC was expressed in Escherichia coli cells transformed by construction that contained fragment of gene coding extracellular domain of receptor for polymeric Ig. MAbs were produced and studied using hybridoma technologies and different methods of immunoenzyme analysis respectively. RESULTS: Panel comprising 10 MAbs against human SC of which 4 types of antibodies recognize cryptic epitopes of free SC and other 6 types recognize epitopes exposed both on SC and sIgA. MAbs panel contains antibodies interacting with conformational and linear epitopes of antigen. Three from obtained MAbs bind to SC epitopes which structure is determined by presence of carbohydrate residues in the molecule of antigen. Immunometric systems were developed which allow to differentially detect free SC and sIgA. CONCLUSION: Developed and characterized MAbs panel recognizing different epitopes of SC molecule opens new opportunities for laboratory and basic research of human secretory immunity.
UR - http://www.scopus.com/inward/record.url?scp=77958174983&partnerID=8YFLogxK
M3 - Article
C2 - 20795387
AN - SCOPUS:77958174983
SP - 54
EP - 59
JO - Журнал микробиологии, эпидемиологии и иммунобиологии
JF - Журнал микробиологии, эпидемиологии и иммунобиологии
SN - 0372-9311
IS - 4
ER -
ID: 89784617