Monitoring of PpIX-induced death dynamics in HeLa cells by digital holographic tomography. / Gorbenko, Daria A.; Belashov, Andrey V.; Litvinov, Ilia K.; Belyaeva, Tatiana N.; Kornilova, Elena S.; Semenova, Irina V.; Vasyutinskii, Oleg S.
Interferometry XX. ed. / Michael B. North Morris; Katherine Creath; Rosario Porras-Aguilar. SPIE, 2020. 1149013 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11490).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
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TY - GEN
T1 - Monitoring of PpIX-induced death dynamics in HeLa cells by digital holographic tomography
AU - Gorbenko, Daria A.
AU - Belashov, Andrey V.
AU - Litvinov, Ilia K.
AU - Belyaeva, Tatiana N.
AU - Kornilova, Elena S.
AU - Semenova, Irina V.
AU - Vasyutinskii, Oleg S.
N1 - Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - We present the analysis of the response of human cervix epidermoid carcinoma HeLa cells to photodynamic treatment with protoporphyrin IX (PpIX) synthesized in living cells under accumulation of 5-aminolevulinic acid (5-ALA). PpIX synthesis was estimated by means of confocal uorescent microscopy basing on its uorescence intensity in the red wavelength range. The cell death dynamics and pathways caused by intracellular generation of reactive oxygen species under laser irradiation of photosensitized cells were examined using digital holographic tomography. These processes were studied both at early stages of cells death on living specimens and at later stages on paraformaldehyde-fixed specimens. Statistical analysis of changes in cellular morphology monitored using holographic tomography allowed us to conclude on cell death pathways, observed at different irradiation doses. Data obtained by holographic tomography have been validated by a standard AO/EB test for cell membrane integrity conducted using the confocal fluorescence microscope.
AB - We present the analysis of the response of human cervix epidermoid carcinoma HeLa cells to photodynamic treatment with protoporphyrin IX (PpIX) synthesized in living cells under accumulation of 5-aminolevulinic acid (5-ALA). PpIX synthesis was estimated by means of confocal uorescent microscopy basing on its uorescence intensity in the red wavelength range. The cell death dynamics and pathways caused by intracellular generation of reactive oxygen species under laser irradiation of photosensitized cells were examined using digital holographic tomography. These processes were studied both at early stages of cells death on living specimens and at later stages on paraformaldehyde-fixed specimens. Statistical analysis of changes in cellular morphology monitored using holographic tomography allowed us to conclude on cell death pathways, observed at different irradiation doses. Data obtained by holographic tomography have been validated by a standard AO/EB test for cell membrane integrity conducted using the confocal fluorescence microscope.
KW - 5-aminolevulinic acid
KW - Cell death pathways
KW - Confocal uorescence microscopy
KW - Digital holographic tomography
KW - Photodynamic treatment
KW - Protoporphyrin IX
UR - http://www.scopus.com/inward/record.url?scp=85092505480&partnerID=8YFLogxK
U2 - 10.1117/12.2567498
DO - 10.1117/12.2567498
M3 - Conference contribution
AN - SCOPUS:85092505480
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Interferometry XX
A2 - North Morris, Michael B.
A2 - Creath, Katherine
A2 - Porras-Aguilar, Rosario
PB - SPIE
T2 - Interferometry XX 2020
Y2 - 24 August 2020 through 4 September 2020
ER -
ID: 76656845