Molecular phylogeny of Paradermamoeba valamo (Amoebozoa, Discosea, Dermamoebida)

Standard

Molecular phylogeny of Paradermamoeba valamo (Amoebozoa, Discosea, Dermamoebida). / Smirnov, Alexey V. ; Kulishkin, Nikita S. ; Surkova, Alina A. ; Mesentsev, Yelisei S. ; Bondarenko, Natalia V. ; Nassonova, Elena S. ; Mazei, Yuri A. .

In: Protistology, Vol. 14, No. 4, 12.2020, p. 219-226.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{10a84c22923549f4958c5b6b17f923b4,

title = "Molecular phylogeny of Paradermamoeba valamo (Amoebozoa, Discosea, Dermamoebida)",

abstract = "A remarkable amoeba species – Paradermamoeba valamo Smirnov et Goodkov,1993 was re-isolated from the bottom sediment in Izmailovo Park pond (Moscow, Russia). For the first time, we obtained modern DIC images of this species. High-quality light-microscopy allowed us to resolve some subcellular structures, including trichocyst-like bodies, and show congruence of the present optical images with the results of earlier electron-microscopic studies. We applied single-cell PCR to obtain full sequence of the 18S rRNA gene of this species. The analysis of genetic distances, as well as the result of phylogenetic analysis, shows that P. valamo is a valid species, related to P. levis but showing clear morphological and molecular differences.",

keywords = "Amoebozoa, Discosea, Light microscopy, Morphology, Phylogeny, SSU gene",

author = "Smirnov, {Alexey V.} and Kulishkin, {Nikita S.} and Surkova, {Alina A.} and Mesentsev, {Yelisei S.} and Bondarenko, {Natalia V.} and Nassonova, {Elena S.} and Mazei, {Yuri A.}",

year = "2020",

month = dec,

doi = "10.21685/1680-0826-2020-14-4-1",

language = "English",

volume = "14",

pages = "219--226",

journal = "Protistology",

issn = "1680-0826",

publisher = "Protozoological Society Affiliated With The Russian Academy Of Sciences",

number = "4",

}

RIS

TY - JOUR

T1 - Molecular phylogeny of Paradermamoeba valamo (Amoebozoa, Discosea, Dermamoebida)

AU - Smirnov, Alexey V.

AU - Kulishkin, Nikita S.

AU - Surkova, Alina A.

AU - Mesentsev, Yelisei S.

AU - Bondarenko, Natalia V.

AU - Nassonova, Elena S.

AU - Mazei, Yuri A.

PY - 2020/12

Y1 - 2020/12

N2 - A remarkable amoeba species – Paradermamoeba valamo Smirnov et Goodkov,1993 was re-isolated from the bottom sediment in Izmailovo Park pond (Moscow, Russia). For the first time, we obtained modern DIC images of this species. High-quality light-microscopy allowed us to resolve some subcellular structures, including trichocyst-like bodies, and show congruence of the present optical images with the results of earlier electron-microscopic studies. We applied single-cell PCR to obtain full sequence of the 18S rRNA gene of this species. The analysis of genetic distances, as well as the result of phylogenetic analysis, shows that P. valamo is a valid species, related to P. levis but showing clear morphological and molecular differences.

AB - A remarkable amoeba species – Paradermamoeba valamo Smirnov et Goodkov,1993 was re-isolated from the bottom sediment in Izmailovo Park pond (Moscow, Russia). For the first time, we obtained modern DIC images of this species. High-quality light-microscopy allowed us to resolve some subcellular structures, including trichocyst-like bodies, and show congruence of the present optical images with the results of earlier electron-microscopic studies. We applied single-cell PCR to obtain full sequence of the 18S rRNA gene of this species. The analysis of genetic distances, as well as the result of phylogenetic analysis, shows that P. valamo is a valid species, related to P. levis but showing clear morphological and molecular differences.

KW - Amoebozoa

KW - Discosea

KW - Light microscopy

KW - Morphology

KW - Phylogeny

KW - SSU gene

UR - http://www.scopus.com/inward/record.url?scp=85101742880&partnerID=8YFLogxK

U2 - 10.21685/1680-0826-2020-14-4-1

DO - 10.21685/1680-0826-2020-14-4-1

M3 - Article

AN - SCOPUS:85101742880

VL - 14

SP - 219

EP - 226

JO - Protistology

JF - Protistology

SN - 1680-0826

IS - 4

ER -

ID: 70801042