TY - JOUR

T1 - Molecular phylogeny and new light microscopic data of Metchnikovella spiralis (Microsporidia

T2 - Metchnikovellidae), a hyperparasite of eugregarine Polyrhabdina sp. from the polychaete Pygospio elegans

AU - Frolova, Ekaterina V.

AU - Paskerova, Gita G.

AU - Smirnov, Alexey V.

AU - Nassonova, Elena S.

N1 - Publisher Copyright: Copyright © The Author(s), 2021. Published by Cambridge University Press.

PY - 2021/6

Y1 - 2021/6

N2 - Metchnikovellids are a deep-branching group of microsporidia, parasites of gregarines inhabiting the alimentary tract of polychaetes and some other invertebrates. The diversity and phylogeny of these hyperparasites remain poorly studied. Modern descriptions and molecular data are still lacking for many species. The results of a light microscopy study and molecular data for Metchnikovella spiralis Sokolova et al. 2014, a hyperparasite of the eugregarine Polyrhabdina sp., isolated from the polychaete Pygospio elegans, were obtained. The original description of M. spiralis was based primarily on the analysis of stained preparations and TEM images. Here, the species description was complemented with the results of in vivo observations and phylogenetic analysis based on the SSU rRNA gene. It was shown that in this species, free sporogony precedes sac-bound sporogony, as it occurs in the life cycle of most other metchnikovellids. Spore sacs are entwined with spirally wound cords, and possess only one polar plug. Phylogenetic analyses did not group M. spiralis with M. incurvata, another metchnikovellid from the same gregarine species, but placed it as a sister branch to Amphiacantha. The paraphyletic nature of the genus Metchnikovella was discussed. The taxonomic summary for M. spiralis was emended.

AB - Metchnikovellids are a deep-branching group of microsporidia, parasites of gregarines inhabiting the alimentary tract of polychaetes and some other invertebrates. The diversity and phylogeny of these hyperparasites remain poorly studied. Modern descriptions and molecular data are still lacking for many species. The results of a light microscopy study and molecular data for Metchnikovella spiralis Sokolova et al. 2014, a hyperparasite of the eugregarine Polyrhabdina sp., isolated from the polychaete Pygospio elegans, were obtained. The original description of M. spiralis was based primarily on the analysis of stained preparations and TEM images. Here, the species description was complemented with the results of in vivo observations and phylogenetic analysis based on the SSU rRNA gene. It was shown that in this species, free sporogony precedes sac-bound sporogony, as it occurs in the life cycle of most other metchnikovellids. Spore sacs are entwined with spirally wound cords, and possess only one polar plug. Phylogenetic analyses did not group M. spiralis with M. incurvata, another metchnikovellid from the same gregarine species, but placed it as a sister branch to Amphiacantha. The paraphyletic nature of the genus Metchnikovella was discussed. The taxonomic summary for M. spiralis was emended.

KW - Gregarines

KW - hyperparasitism

KW - parasites

KW - Metchnikovellidae

KW - polychaetes

KW - Gregarines

KW - Hyperparasitism

KW - Intracellular parasites

KW - Light microscopy

KW - Metchnikovellidae

KW - Microsporidia

KW - Molecular phylogeny

KW - Polychaetes

KW - intracellular parasites

KW - microsporidia

KW - hyperparasitism

KW - molecular phylogeny

KW - polychaetes

KW - light microscopy

KW - REDESCRIPTION

KW - MESNIL

KW - SEQUENCE ALIGNMENT

KW - CAULLERY

KW - ULTRASTRUCTURE

KW - PARASITE

UR - http://www.scopus.com/inward/record.url?scp=85104338927&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/88a31463-1a3c-3407-9bcd-0416c0ab494c/

U2 - 10.1017/s0031182021000603

DO - 10.1017/s0031182021000603

M3 - Article

C2 - 33843504

AN - SCOPUS:85104338927

VL - 148

SP - 779

EP - 786

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 7

ER -