TY - JOUR

T1 - Molecular Phylogenetics and Light Microscopy Reveal “True” and “False” Calacarines and Novel Genital Structures in Gall Mites (Acariformes, Eriophyoidea)

AU - Chetverikov, P.E.

AU - Craemer, C.

AU - Gankevich, V.D.

AU - Le, N.T.T.

AU - Nguyen, V.D.

AU - Trinh, H.X.

AU - Amrine, J.

N1 - Export Date: 4 March 2024 Адрес для корреспонденции: Chetverikov, P.E.; Department of Invertebrate Zoology, Universitetskaya Naberezhnaya 7/9, Russian Federation; эл. почта: philipp-chetverikov@yandex.ru Сведения о финансировании: Deutsche Forschungsgemeinschaft, DFG, SFB 992/1 2012 Сведения о финансировании: Bundesministerium für Bildung und Forschung, BMBF, 031 A538A/A538C RBC, 031L0106 de.STAIR Сведения о финансировании: Ministry of Education and Science of the Russian Federation, Minobrnauka, 075-15-2022-322 Текст о финансировании 1: This research was funded by Ministry of Science and Higher Education of the Russian Federation (agreement 075-15-2022-322 “Agricultural technologies for the Future”). Текст о финансировании 2: PCR and sequencing were conducted with the equipment of the “Development of Molecular and Cellular Technologies”, “The Bio-Bank”, and “Microscopy and Microanalysis” Resource Centers of St. Petersburg State University (Russia). We thank A. Kudryavtzev (St. Petersburg State Agrarian University, Russia) for his assistance in slide-mounting and measuring calacarines. The Galaxy server that was used for some calculations is in part funded by Collaborative Research Centre 992 Medical Epigenetics (DFG grant SFB 992/1 2012) and German Federal Ministry of Education and Research (BMBF grants 031 A538A/A538C RBC, 031L0101B/031L0101C de.NBI-epi, 031L0106 de.STAIR (de.NBI)). 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PY - 2024/2/8

Y1 - 2024/2/8

N2 - Gall mites (Eriophyoidea) are cosmopolitan microscopic phytoparasites that often transmit viruses and induce gallogenesis. The tribe Calacarini is diagnosed by a set of plesiomorphic and homoplastic traits, including elimination of setae sc shared with other lineages of Eriophyoidea. We reviewed data on the generic diversity of calacarines, revised the concept of the type genus Calacarus Keifer 1940, and proposed three zones (MZ, SMZ, LZ) in the prodorsal shields of calacarines to simplify descriptions of their shield patterns. We describe three new calacarine species (Calacarus baviensis n. sp., C. burchelliae n. sp., and Viginticus searsiae n. sp.) from indigenous dicotyledonous trees from South Africa and Vietnam and report on new findings of Paracalacarus podocarpi Keifer in Brazil, Jiangsuacarus sp. in the USA, and Calacarus pusillus Pye in Latvia and Russia. The latter represents the new most northern locality of Calacarini. Reinvestigating the type species of Jaranasia Chandrapatya & Boczek 2000 revealed that absence of setae l’’ II is the only character separating it from Jiangsuacarus Xue 2009. We proposed two new combinations: Jiangsuacarus sesleriae (Skoracka 2004) n. comb. (transferred from Jaranasia) and Procalacarus mussaendae (Keifer 1977) n. comb. (transferred from Calacarus). Partial sequences of Cox1 and 28S genes were obtained for six calacarines, some of them originating from old ethanol material kept at room temperature. Molecular phylogenetics revealed a stable cluster of “true” calacarine sequences comprising Calacarus, Jaranasia, Latitudo, and Viginticus and a polyphyletic group of erroneous sequences assigned to Calacarini in GenBank. All investigated females of calacarines have a pair of genital tubules associated with the vestibulum and hypothesized to participate in fertilization. This finding may contribute to resolving the question on how the fusion of gametes happens in gall mites. © 2024 by the authors.

AB - Gall mites (Eriophyoidea) are cosmopolitan microscopic phytoparasites that often transmit viruses and induce gallogenesis. The tribe Calacarini is diagnosed by a set of plesiomorphic and homoplastic traits, including elimination of setae sc shared with other lineages of Eriophyoidea. We reviewed data on the generic diversity of calacarines, revised the concept of the type genus Calacarus Keifer 1940, and proposed three zones (MZ, SMZ, LZ) in the prodorsal shields of calacarines to simplify descriptions of their shield patterns. We describe three new calacarine species (Calacarus baviensis n. sp., C. burchelliae n. sp., and Viginticus searsiae n. sp.) from indigenous dicotyledonous trees from South Africa and Vietnam and report on new findings of Paracalacarus podocarpi Keifer in Brazil, Jiangsuacarus sp. in the USA, and Calacarus pusillus Pye in Latvia and Russia. The latter represents the new most northern locality of Calacarini. Reinvestigating the type species of Jaranasia Chandrapatya & Boczek 2000 revealed that absence of setae l’’ II is the only character separating it from Jiangsuacarus Xue 2009. We proposed two new combinations: Jiangsuacarus sesleriae (Skoracka 2004) n. comb. (transferred from Jaranasia) and Procalacarus mussaendae (Keifer 1977) n. comb. (transferred from Calacarus). Partial sequences of Cox1 and 28S genes were obtained for six calacarines, some of them originating from old ethanol material kept at room temperature. Molecular phylogenetics revealed a stable cluster of “true” calacarine sequences comprising Calacarus, Jaranasia, Latitudo, and Viginticus and a polyphyletic group of erroneous sequences assigned to Calacarini in GenBank. All investigated females of calacarines have a pair of genital tubules associated with the vestibulum and hypothesized to participate in fertilization. This finding may contribute to resolving the question on how the fusion of gametes happens in gall mites. © 2024 by the authors.

KW - 28S

KW - Acari

KW - arthropod structure

KW - Calacarus

KW - Cox1

KW - endemic

KW - erroneous sequences

KW - female genitalia

KW - phytoparasite diversity

KW - phytophagous mite

KW - reproductive system

KW - 28s

KW - Arthropod structure

KW - Calacari

KW - Endemic

KW - Erroneous sequence

KW - Female genitalia

KW - Phytoparasite diversity

KW - Phytophagous mite

KW - Reproductive systems

KW - Viruses

KW - Arachnids

KW - Genes

KW - Microscopy

KW - Patterns

KW - Sequences

KW - Trees

KW - Type

UR - https://www.mendeley.com/catalogue/6559f160-0105-3bb9-a717-a69774171a01/

U2 - 10.3390/f15020329

DO - 10.3390/f15020329

M3 - статья

VL - 15

JO - Forests

JF - Forests

SN - 1999-4907

IS - 2

M1 - 329

ER -