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Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes. / Shirmanova, Marina V.; Shcheslavskiy, Vladislav I.; Lukina, Maria M.; Dudenkova, Varvara V.; Kritchenkov, Ilya S.; Solomatina, Anastasia I.; Tunik, Sergey P.

Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis. ed. / Robert R. Alfano; Stavros G. Demos; Angela B. Seddon. SPIE, 2020. 112340G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11234).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Harvard

Shirmanova, MV, Shcheslavskiy, VI, Lukina, MM, Dudenkova, VV, Kritchenkov, IS, Solomatina, AI & Tunik, SP 2020, Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes. in RR Alfano, SG Demos & AB Seddon (eds), Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis., 112340G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11234, SPIE, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis 2020, San Francisco, United States, 2/02/20. https://doi.org/10.1117/12.2549248

APA

Shirmanova, M. V., Shcheslavskiy, V. I., Lukina, M. M., Dudenkova, V. V., Kritchenkov, I. S., Solomatina, A. I., & Tunik, S. P. (2020). Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes. In R. R. Alfano, S. G. Demos, & A. B. Seddon (Eds.), Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis [112340G] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11234). SPIE. https://doi.org/10.1117/12.2549248

Vancouver

Shirmanova MV, Shcheslavskiy VI, Lukina MM, Dudenkova VV, Kritchenkov IS, Solomatina AI et al. Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes. In Alfano RR, Demos SG, Seddon AB, editors, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis. SPIE. 2020. 112340G. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2549248

Author

Shirmanova, Marina V. ; Shcheslavskiy, Vladislav I. ; Lukina, Maria M. ; Dudenkova, Varvara V. ; Kritchenkov, Ilya S. ; Solomatina, Anastasia I. ; Tunik, Sergey P. / Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes. Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis. editor / Robert R. Alfano ; Stavros G. Demos ; Angela B. Seddon. SPIE, 2020. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

BibTeX

@inproceedings{fbecfb08bf5c43199416569d8ee94a3d,
title = "Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes",
abstract = "Non-invasive optical techniques on the basis of quenching of phosphorescence by molecular oxygen represent a powerful tool to perform mapping of oxygen content in biological systems. The development of phosphorescent oxygensensitive probes suitable for biological applications, especially in vivo, remains a challenging task. In this paper we applied for biological investigations one novel and one previously published Ir(III)-based complexes. This complexes demonstrate low cytotoxicity at the concentrations ≤70 μM and good uptake by cultured cancer cells. Both compounds were primarily located within the cytoplasm. Using TCSPC-based phosphorescence lifetime macro-imaging in vivo, we found that IR-2 complex shows detectable phosphorescence in tumor tissue in mice upon local injection. These results illustrate the high potential of organometallic complexes under study for mapping of oxygen level in cells and tissues.",
keywords = "Cancer, Ir(III)-based complexes, Oxygen, Phosphorescence Lifetime Imaging, PLIM, IN-VITRO, PHOSPHORESCENT, IRIDIUM(III) COMPLEXES, cancer, oxygen, PROBES",
author = "Shirmanova, {Marina V.} and Shcheslavskiy, {Vladislav I.} and Lukina, {Maria M.} and Dudenkova, {Varvara V.} and Kritchenkov, {Ilya S.} and Solomatina, {Anastasia I.} and Tunik, {Sergey P.}",
year = "2020",
month = feb,
day = "21",
doi = "10.1117/12.2549248",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Alfano, {Robert R.} and Demos, {Stavros G.} and Seddon, {Angela B.}",
booktitle = "Optical Biopsy XVIII",
address = "United States",
note = "Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis 2020 ; Conference date: 02-02-2020 Through 05-02-2020",

}

RIS

TY - GEN

T1 - Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes

AU - Shirmanova, Marina V.

AU - Shcheslavskiy, Vladislav I.

AU - Lukina, Maria M.

AU - Dudenkova, Varvara V.

AU - Kritchenkov, Ilya S.

AU - Solomatina, Anastasia I.

AU - Tunik, Sergey P.

PY - 2020/2/21

Y1 - 2020/2/21

N2 - Non-invasive optical techniques on the basis of quenching of phosphorescence by molecular oxygen represent a powerful tool to perform mapping of oxygen content in biological systems. The development of phosphorescent oxygensensitive probes suitable for biological applications, especially in vivo, remains a challenging task. In this paper we applied for biological investigations one novel and one previously published Ir(III)-based complexes. This complexes demonstrate low cytotoxicity at the concentrations ≤70 μM and good uptake by cultured cancer cells. Both compounds were primarily located within the cytoplasm. Using TCSPC-based phosphorescence lifetime macro-imaging in vivo, we found that IR-2 complex shows detectable phosphorescence in tumor tissue in mice upon local injection. These results illustrate the high potential of organometallic complexes under study for mapping of oxygen level in cells and tissues.

AB - Non-invasive optical techniques on the basis of quenching of phosphorescence by molecular oxygen represent a powerful tool to perform mapping of oxygen content in biological systems. The development of phosphorescent oxygensensitive probes suitable for biological applications, especially in vivo, remains a challenging task. In this paper we applied for biological investigations one novel and one previously published Ir(III)-based complexes. This complexes demonstrate low cytotoxicity at the concentrations ≤70 μM and good uptake by cultured cancer cells. Both compounds were primarily located within the cytoplasm. Using TCSPC-based phosphorescence lifetime macro-imaging in vivo, we found that IR-2 complex shows detectable phosphorescence in tumor tissue in mice upon local injection. These results illustrate the high potential of organometallic complexes under study for mapping of oxygen level in cells and tissues.

KW - Cancer

KW - Ir(III)-based complexes

KW - Oxygen

KW - Phosphorescence Lifetime Imaging

KW - PLIM

KW - IN-VITRO

KW - PHOSPHORESCENT

KW - IRIDIUM(III) COMPLEXES

KW - cancer

KW - oxygen

KW - PROBES

UR - http://www.scopus.com/inward/record.url?scp=85082719973&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/b4faeda3-004f-3509-93c8-454b62099159/

U2 - 10.1117/12.2549248

DO - 10.1117/12.2549248

M3 - Conference contribution

AN - SCOPUS:85082719973

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optical Biopsy XVIII

A2 - Alfano, Robert R.

A2 - Demos, Stavros G.

A2 - Seddon, Angela B.

PB - SPIE

T2 - Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis 2020

Y2 - 2 February 2020 through 5 February 2020

ER -

ID: 53243493