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Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI). / Zagata, P.; Greczek-Stachura, M.; Tarcz, S.; Rautian, M.

In: FOLIA BIOLOGICA-KRAKOW, Vol. 63, No. 1, 2015, p. 1-7.

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@article{d503f5c294674ecc98c1feb034978083,
title = "Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI)",
abstract = "Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen",
keywords = "Paramecium bursaria, syngen, phylogeny",
author = "P. Zagata and M. Greczek-Stachura and S. Tarcz and M. Rautian",
year = "2015",
doi = "10.3409/fb63_l.77",
language = "English",
volume = "63",
pages = "1--7",
journal = "FOLIA BIOLOGICA-KRAKOW",
issn = "0015-5497",
publisher = "Institute of Systematics and Evolution of Animals",
number = "1",

}

RIS

TY - JOUR

T1 - Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI)

AU - Zagata, P.

AU - Greczek-Stachura, M.

AU - Tarcz, S.

AU - Rautian, M.

PY - 2015

Y1 - 2015

N2 - Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen

AB - Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen

KW - Paramecium bursaria

KW - syngen

KW - phylogeny

U2 - 10.3409/fb63_l.77

DO - 10.3409/fb63_l.77

M3 - Article

VL - 63

SP - 1

EP - 7

JO - FOLIA BIOLOGICA-KRAKOW

JF - FOLIA BIOLOGICA-KRAKOW

SN - 0015-5497

IS - 1

ER -

ID: 3976051