Research output: Contribution to journal › Article
Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI). / Zagata, P.; Greczek-Stachura, M.; Tarcz, S.; Rautian, M.
In: FOLIA BIOLOGICA-KRAKOW, Vol. 63, No. 1, 2015, p. 1-7.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI)
AU - Zagata, P.
AU - Greczek-Stachura, M.
AU - Tarcz, S.
AU - Rautian, M.
PY - 2015
Y1 - 2015
N2 - Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen
AB - Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen
KW - Paramecium bursaria
KW - syngen
KW - phylogeny
U2 - 10.3409/fb63_l.77
DO - 10.3409/fb63_l.77
M3 - Article
VL - 63
SP - 1
EP - 7
JO - FOLIA BIOLOGICA-KRAKOW
JF - FOLIA BIOLOGICA-KRAKOW
SN - 0015-5497
IS - 1
ER -
ID: 3976051