Molecular identification of a densovirus in healthy and diseased zophobas morio (coleoptera, tenebrionidae)

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Molecular identification of a densovirus in healthy and diseased zophobas morio (coleoptera, tenebrionidae). / Tokarev, Yuri S.; Malysh, Svetlana M.; Volodartseva, Yuliya V.; Gerus, Aleksei V.; Berezin, Mikhail V.

In: Intervirology, Vol. 62, No. 5-6, 01.07.2020, p. 222-226.

Research output: Contribution to journal › Article › peer-review

Author

Tokarev, Yuri S. ; Malysh, Svetlana M. ; Volodartseva, Yuliya V. ; Gerus, Aleksei V. ; Berezin, Mikhail V. / Molecular identification of a densovirus in healthy and diseased zophobas morio (coleoptera, tenebrionidae). In: Intervirology. 2020 ; Vol. 62, No. 5-6. pp. 222-226.

BibTeX

@article{4d9d49343e5c4009900abf3112314b06,

title = "Molecular identification of a densovirus in healthy and diseased zophobas morio (coleoptera, tenebrionidae)",

abstract = "Zophobas morio is a tropical darkling beetle which is widely exploited for commercial large-scale insect growing. Outbreaks of a disease may occur causing total devastation of cultures. In the present paper, samples of diseased Z. morio were obtained and used for establishment of a laboratory model as they were found infective to the larvae of the same insect species from another source. It took about 1 month to develop symptoms of acute disease in mid-Age larvae and about twice as much when younger larvae were used for infection. Affected larvae perished quickly, and within several days up to 90-100% of the colony could perish. Both in healthy and diseased larvae a virus was detected using PCR with degenerate primers specific for a gene coding for a non-structural protein (ORF3). The sequenced gene fragment (Genbank accession #MN732869) confirmed allocation of the virus to Densoviridae, with maximal similarity of 97.2% to Blatella germanica densovirus-like virus (#JQ320376) and 66.2% to B. germanica densovirus (#AY189948). Genomic DNA samples of Z. morio larvae from an independent colony devoid of symptoms of a disease were also positive for this virus with a slightly different (99.7% sequence similarity to the former sequence of the Z. morio densovirus) genotype (#MN732870). ",

author = "Tokarev, {Yuri S.} and Malysh, {Svetlana M.} and Volodartseva, {Yuliya V.} and Gerus, {Aleksei V.} and Berezin, {Mikhail V.}",

note = "Publisher Copyright: {\textcopyright} 2020 ",

year = "2020",

month = jul,

day = "1",

doi = "10.1159/000508839",

language = "English",

volume = "62",

pages = "222--226",

journal = "Intervirology",

issn = "0300-5526",

publisher = "S. Karger AG",

number = "5-6",

}

RIS

TY - JOUR

T1 - Molecular identification of a densovirus in healthy and diseased zophobas morio (coleoptera, tenebrionidae)

AU - Tokarev, Yuri S.

AU - Malysh, Svetlana M.

AU - Volodartseva, Yuliya V.

AU - Gerus, Aleksei V.

AU - Berezin, Mikhail V.

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Zophobas morio is a tropical darkling beetle which is widely exploited for commercial large-scale insect growing. Outbreaks of a disease may occur causing total devastation of cultures. In the present paper, samples of diseased Z. morio were obtained and used for establishment of a laboratory model as they were found infective to the larvae of the same insect species from another source. It took about 1 month to develop symptoms of acute disease in mid-Age larvae and about twice as much when younger larvae were used for infection. Affected larvae perished quickly, and within several days up to 90-100% of the colony could perish. Both in healthy and diseased larvae a virus was detected using PCR with degenerate primers specific for a gene coding for a non-structural protein (ORF3). The sequenced gene fragment (Genbank accession #MN732869) confirmed allocation of the virus to Densoviridae, with maximal similarity of 97.2% to Blatella germanica densovirus-like virus (#JQ320376) and 66.2% to B. germanica densovirus (#AY189948). Genomic DNA samples of Z. morio larvae from an independent colony devoid of symptoms of a disease were also positive for this virus with a slightly different (99.7% sequence similarity to the former sequence of the Z. morio densovirus) genotype (#MN732870).

AB - Zophobas morio is a tropical darkling beetle which is widely exploited for commercial large-scale insect growing. Outbreaks of a disease may occur causing total devastation of cultures. In the present paper, samples of diseased Z. morio were obtained and used for establishment of a laboratory model as they were found infective to the larvae of the same insect species from another source. It took about 1 month to develop symptoms of acute disease in mid-Age larvae and about twice as much when younger larvae were used for infection. Affected larvae perished quickly, and within several days up to 90-100% of the colony could perish. Both in healthy and diseased larvae a virus was detected using PCR with degenerate primers specific for a gene coding for a non-structural protein (ORF3). The sequenced gene fragment (Genbank accession #MN732869) confirmed allocation of the virus to Densoviridae, with maximal similarity of 97.2% to Blatella germanica densovirus-like virus (#JQ320376) and 66.2% to B. germanica densovirus (#AY189948). Genomic DNA samples of Z. morio larvae from an independent colony devoid of symptoms of a disease were also positive for this virus with a slightly different (99.7% sequence similarity to the former sequence of the Z. morio densovirus) genotype (#MN732870).

UR - http://www.scopus.com/inward/record.url?scp=85087527752&partnerID=8YFLogxK

U2 - 10.1159/000508839

DO - 10.1159/000508839

M3 - Article

C2 - 32594081

AN - SCOPUS:85087527752

VL - 62

SP - 222

EP - 226

JO - Intervirology

JF - Intervirology

SN - 0300-5526

IS - 5-6

ER -

ID: 98190039