Research output: Contribution to journal › Article › peer-review
Microextraction of Tetracyclines from Milk to Deep Eutectic Solvents for the Subsequent Determination by High-Performance Liquid Chromatography–Tandem Mass Spectrometry. / Cherkashina, K. D. ; Pochivalov, A. S. ; Shakirova, F. M. ; Shishov, A. Yu. ; Bulatov, A. V. .
In: Journal of Analytical Chemistry, Vol. 77, No. 3, 01.03.2022, p. 334-341.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Microextraction of Tetracyclines from Milk to Deep Eutectic Solvents for the Subsequent Determination by High-Performance Liquid Chromatography–Tandem Mass Spectrometry
AU - Cherkashina, K. D.
AU - Pochivalov, A. S.
AU - Shakirova, F. M.
AU - Shishov, A. Yu.
AU - Bulatov, A. V.
N1 - Publisher Copyright: © 2022, Pleiades Publishing, Ltd.
PY - 2022/3/1
Y1 - 2022/3/1
N2 - A method of liquid–liquid microextraction is proposed for the extraction and preconcentration of tetracycline antibiotics from milk. It involves the extraction of analytes into a hydrophobic deep eutectic solvent (DES) based on a terpenoid and a carboxylic acid, followed by back extraction into the aqueous phase. A synergistic effect was revealed in the microextraction of tetracyclines into a DES phase based on thymol and octanoic acid (molar ratio of precursors 0.35 : 0.65), i.e., higher distribution ratios compared to those calculated using the additive scheme. Possibilities of the proposed method are shown on an example of the determination of tetracycline, chlortetracycline, and doxycycline in milk samples by HPLC–MS/MS. The limits of detection (3σ) for tetracycline and doxycycline are 5 ng/kg and for chlortetracycline,—50 ng/kg.
AB - A method of liquid–liquid microextraction is proposed for the extraction and preconcentration of tetracycline antibiotics from milk. It involves the extraction of analytes into a hydrophobic deep eutectic solvent (DES) based on a terpenoid and a carboxylic acid, followed by back extraction into the aqueous phase. A synergistic effect was revealed in the microextraction of tetracyclines into a DES phase based on thymol and octanoic acid (molar ratio of precursors 0.35 : 0.65), i.e., higher distribution ratios compared to those calculated using the additive scheme. Possibilities of the proposed method are shown on an example of the determination of tetracycline, chlortetracycline, and doxycycline in milk samples by HPLC–MS/MS. The limits of detection (3σ) for tetracycline and doxycycline are 5 ng/kg and for chlortetracycline,—50 ng/kg.
KW - liquid–liquid microextraction
KW - deep eutectic solvents
KW - tetracyclines
KW - food products
KW - High-performance liquid chromatography
KW - high-performance liquid chromatography
UR - https://link.springer.com/article/10.1134/S1061934822030042
UR - http://www.scopus.com/inward/record.url?scp=85127511829&partnerID=8YFLogxK
U2 - 10.1134/s1061934822030042
DO - 10.1134/s1061934822030042
M3 - Article
VL - 77
SP - 334
EP - 341
JO - Journal of Analytical Chemistry
JF - Journal of Analytical Chemistry
SN - 1061-9348
IS - 3
ER -
ID: 94819197