Research output: Contribution to journal › Review article › peer-review
Methodological approaches for studying the european water frog Pelophylax esculentus complex. / Dedukh, D. V.; Krasikova, A. V.
In: Russian Journal of Genetics, Vol. 53, No. 8, 01.08.2017, p. 843-850.Research output: Contribution to journal › Review article › peer-review
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TY - JOUR
T1 - Methodological approaches for studying the european water frog Pelophylax esculentus complex
AU - Dedukh, D. V.
AU - Krasikova, A. V.
PY - 2017/8/1
Y1 - 2017/8/1
N2 - The European water frog (Pelophylax esculentus) complex represents a unique and adequate model system for the study of interspecific hybridization and the mechanisms enabling interspecific hybrids to overcome the reproductive barriers. The difficulties in the study of individuals from the P. esculentus complex are associated with high polymorphism of morphological characters in parental species and interspecific hybrids, as well as with the presence of polyploid hybrid forms. From the discovery of the phenomenon of interspecific hybridization and the demonstration of successful reproduction of interspecific hybrids, researchers constantly searched for the methods necessary for the most accurate identification of parental species and various hybrid forms. This review describes biochemical, cytogenetic, and molecular methods and approaches used to identify individuals from the European water frog complex, as well as to analyze the genomes transferred with the gametes of hybrids. The advantages and disadvantages of these approaches are discussed. The presented methods can be used for studying other hybrid complexes of fish, amphibians, and reptiles.
AB - The European water frog (Pelophylax esculentus) complex represents a unique and adequate model system for the study of interspecific hybridization and the mechanisms enabling interspecific hybrids to overcome the reproductive barriers. The difficulties in the study of individuals from the P. esculentus complex are associated with high polymorphism of morphological characters in parental species and interspecific hybrids, as well as with the presence of polyploid hybrid forms. From the discovery of the phenomenon of interspecific hybridization and the demonstration of successful reproduction of interspecific hybrids, researchers constantly searched for the methods necessary for the most accurate identification of parental species and various hybrid forms. This review describes biochemical, cytogenetic, and molecular methods and approaches used to identify individuals from the European water frog complex, as well as to analyze the genomes transferred with the gametes of hybrids. The advantages and disadvantages of these approaches are discussed. The presented methods can be used for studying other hybrid complexes of fish, amphibians, and reptiles.
KW - comparative genomic hybridization
KW - European water frogs
KW - fluorescent in situ hybridization
KW - genomic in situ hybridization
KW - interspecific hybridization
KW - polyploid forms
UR - http://www.scopus.com/inward/record.url?scp=85028743962&partnerID=8YFLogxK
U2 - 10.1134/S102279541708004X
DO - 10.1134/S102279541708004X
M3 - Review article
AN - SCOPUS:85028743962
VL - 53
SP - 843
EP - 850
JO - Russian Journal of Genetics
JF - Russian Journal of Genetics
SN - 1022-7954
IS - 8
ER -
ID: 35967621